摘要
[目的]研究猪传染性胃肠炎病毒S1融合蛋白的免疫原性。[方法]表达和纯化了猪传染性胃肠炎病毒S1与TAT转导肽序列的融合蛋白S1-TAT,将融合蛋白S1-TAT经腹腔注射和灌胃方式免疫昆明小鼠,采集免疫小鼠血液和粪便样品,检测血清Ig G抗体和黏膜Ig A抗体水平,期间观察小鼠体重变化。[结果]腹腔注射组诱导产生的血清Ig G抗体水平明显高于其他试验组(P<0.01),但其黏膜Ig A抗体水平较低;而灌胃组能够诱导中等水平的血清Ig G抗体和较高水平的黏膜Ig A抗体(P<0.01);在试验周期中,各组小鼠体重均正常。融合蛋白S1-TAT的穿肠活性较好,与对照组之间差异极显著(P<0.01)。[结论]融合蛋白S1-TAT经口服或注射小鼠后,能够有效诱导小鼠机体产生体液免疫应答,并且融合蛋白S1-TAT经口服可诱导小鼠机体产生黏膜免疫应答;融合蛋白S1-TAT具有穿肠功能;融合蛋白S1-TAT经口服或注射小鼠,均安全。
[Objective] To investigate the immunogenicity of fusion protein S1-TAT consisting of transmissible gastroenteritis virus S1 and transcriptional activator protein（ TAT）. [Method] The recombinant plasmid S1-TAT was expressed in prokaryotic system and purified. Kunming mice were immunized with fusion protein S1-TAT by intraperitoneal injection and intragastric administration respectively,collected the blood and stool samples of mice and determined for serum Ig G and mucosal Ig A antibody,observed the change of bodyweight. [Result]The level of serum Ig G antibody of intraperitoneal injection group was significantly higher than other groups（ P 0. 01）,but mucosal Ig A antibody was lower. However,the intragastric administration group induced a moderate level of serum Ig G antibody and higher level of mucosal Ig A antibody（ P 0. 01）.Throughout the immune process,the bodyweight of each group was within the normal range. The intestine activity of fusion protein S1-TAT was better compared with the control group（ P 0. 01）. [Conclusion] The fusion protein S1-TAT can effectively induce the humoral immune response in mice after oral administration or injection,and the mucosal immune response can be induced by oral administration. The fusion protein S1-TAT has intestinal function. It is safe to immune mice by oral administration or injection.
作者
王芳
徐进平
WANG Fang,XU Jin-ping(State Key Lab of Viral,School of Life Seienee,Wuhan University,Wuhan,Hubei 43007)
出处
《安徽农业科学》
CAS
2018年第13期104-107,169,共5页
Journal of Anhui Agricultural Sciences
关键词
猪传染性胃肠炎病毒S1基因
转导肽
免疫原性
Transmissible gastroenteritis virus S1 gene
Transduction peptide
Immunogenicity
