摘要
为研究菲律宾蛤仔Ruditapes philippinarum早期发育阶段对总氨态氮(TAN)和非离子氨(UIA)的耐受性,在水温为21~24℃、pH为7.9~8.3、盐度为27~30条件下,开展了TAN对菲律宾蛤仔受精卵、D形幼虫(壳长为103.2μm±3.0μm)和稚贝(壳长为318.1μm±27.3μm)的急性毒性试验。结果表明:TAN对菲律宾蛤仔受精卵孵化率的24 h EC_(50)为7.29 mg/L(UIA浓度为0.502 mg/L);对D形幼虫死亡率的96 h LC_(50)为7.94 mg/L(UIA浓度为0.212 mg/L);对稚贝死亡率的96 h LC_(50)为49.0 mg/L(UIA浓度为2.10 mg/L),对稚贝壳长相对生长的96 h EC_(50)为4.9 mg/L(UIA浓度为0.21 mg/L);对稚贝壳高相对生长的96 h EC_(50)为10.5 mg/L(UIA浓度为0.448 mg/L);菲律宾蛤仔对TAN的耐受能力为稚贝>D形幼虫。研究表明,菲律宾蛤仔育苗期间非离子氨浓度控制在0.020 mg/L以内较好。
Acute toxicity test of total ammonia nitrogen（ TAN） level（ 0. 167-29. 6 mg/L,non-ionic ammonia,UIA,0.012-2.03 mg/L） on fertilized eggs,D-larvae with body length of（ 103.2±3.0） μm and juveniles with body length of（ 318.1±27.3） μm in Manila clam Ruditapes philippinarum was conducted at water temperature of 21-24℃,pH 7.9-8.3,and a salinity of 27-30 in order to investigate the tolerance of Manila clam to TAN at early development. It was found that the 24 h effective concentration（ EC50） of TAN on hatching rate was 7.29 mg/L（ UIA0.502 mg/L） in fertilized eggs and 96 h half lethal concentration（ 96 h LC50） was 7.94 mg/L（ UIA 0.212 mg/L）for D-larvae and 49.0 mg/L（ UIA 2.10 mg/L） for juveniles. The 96 h EC50 was 4.9 mg/L（ UIA,0.21 mg/L） for relative growth of juveniles for shell length and 10.5 mg/L（ UIA 0.448 mg/L） for shell height. The order of tolerance of Manila clam to ammonia nitrogen level was expressed as juvenile D-larvae,suggesting that UIA should be controlled at below 0.020 mg/L during breeding.
作者
韩天坤
杨凤
贾甲
李晓旭
闫喜武
HAN Tian-kun;YANG F e n g;JIA Jia;LI Xiao-x u;Y A N X i-w u(College of Fisheries and Life Science, Dalian Ocean Lniversity,Dalian 116023,China;Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province,Dalian 116023,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2018年第2期210-216,共7页
Journal of Dalian Ocean University
基金
现代农业产业技术体系建设专项(CARS-49)
关键词
总氨态氮
菲律宾蛤仔
早期生长发育
total ammonia nitrogen
Ruditapes philippinarum
early growth and development
