摘要
目的观察染尘后矽肺性肺纤维化大鼠高迁移率族蛋白B1(HMGB1)的表达以及地龙对大鼠的干预作用。方法将45只大鼠根据随机数发生器产生的随机数序列分为空白组、模型组和地龙组,每组15只。模型组和地龙组利用硅尘建立大鼠肺纤维化模型,空白组气管内注入生理盐水。地龙组用地龙煎液4 ml/kg灌胃,空白组和模型组用等量生理盐水灌胃。各组分别在第7、14、28天处死5只大鼠,测定肺系数,将肺组织行常规HE染色,镜下比较3组肺泡炎和肺纤维化程度;并采用免疫组化法观察HMGB1的表达。结果模型组第7、14、28天肺系数(mg/g)分别为6.48±0.19、6.71±0.14、7.01±0.33,均较空白组(4.36±0.13、4.52±0.33、4.56±0.10)高(均P<0.01);地龙组第7、14、28天肺系数(mg/g)分别为5.07±0.17、5.38±0.05、5.53±0.08,均较同时间点模型组低(均P<0.05);模型组第7、14、28天HMGB1光密度平均值分别为0.31±0.03、0.35±0.03、0.38±0.02,均较空白组(0.20±0.02、0.21±0.04、0.20±0.03)增高(均P<0.01);地龙组第7、14、28天HMGB1光密度平均值分别为0.30±0.02、0.29±0.02、0.27±0.03,与模型组比较,第7天差异无统计学意义(P=0.507),第14、28天均明显下调(均P<0.05)。结论染尘后大鼠出现肺纤维化改变,HMGB1表达进行性增高,而地龙干预后肺纤维化程度减轻,这可能与HMGB1表达受抑制有关。
Objective To observe the expression of high mobility group protein B1 （ HMGB1 ） in the lung tissues of silic- otic fibrosis rats, and explore the effectiveness of TCM Lumbricus medicine intervention. Methods A total of 45 rats were randomly divided into control group, model group and Lumbricus group with 15 rats in each group. The rats in mod- el group and Lumbricus group received silica exposure to develop a silicotic fibrosis rat model, while the control group re- ceived intratracheal instillation of saline. The Lumbricus group received earthworm decoction 4 ml/kg by gavage, while the control group and model group received the same volume of normal saline. Five rats in each group were sacrificed on 7th, 14th and 28th days at a time. The lung coefficient was measured. The development of alveolitis and pulmonary fibro- sis were compared among the three groups. The expression of HMGB1 was observed by immunohistochemistry. Results The lung coefficient of model group on the 7th, 14th and 28th days was 6.48±0.19, 6.71 ± 0. 14, 7.01 ± 0.33, re- spectively, which were increased as compared with the control group （4.36±0.13, 4.52 ±0.33, 4.56 ±0.10）, re- spectively, all P〈0.01 ; The lung coefficient of Lumbricus group on the 7th, 14th and 28th days was 5.07 ±0. 17, 5.38 ± 0.05, 5.53 ± 0.08, respectively, which was decreased as compared with model group, P 〈 0.01; The optical density of HMGB1 of model group on the 7th, 14th and 28th days was 0.31 ±0.03, 0.35± 0.03, 0.38± 0.02, re- spectively, which was increased as compared with control group （0.20 -＋ 0.02, 0.21 ± 0.04, 0.20 ± 0.03 ）, P 〈 0. 01 ; The optical density of HMGB1 of the Lumbricus group on the 7th, 14th and 28th days was 0.30 ± 0.02, 0.29± 0.02, 0. 27 ± 0.03, respectively, the difference on the 7th days was not significant as compared with the model group （ P = 0.507）, but decreased obviously on the 14th and 28th days （P 〈 0.05）. Conclusion The pulmonary fibrosis and progressive elevated HMGB1 can be observed in rats after exposure to dust, which can be improved by TCM Lumbricus medicine intervention, the mechanism may associate with inhibition of TCM Lumbricus on the expression of HMGB1.
作者
喻婷
李国辉
贾仰民
陈素珍
严伟伟
娄懿
何飞
YU Ting;LI Guo-hui;JIA Yang-min(Department of Occupational Diseases, Zhejiang Combination Hospital of Traditional Chinese medicine and Western Medicine, Hangzhou , Zhejiang 310053, China)
出处
《中华全科医学》
2018年第5期697-700,707,共5页
Chinese Journal of General Practice
基金
国家自然基金青年科学基金项目(81403336)
浙江省中管局科研基金项目(2015ZA144)
关键词
地龙
矽肺
高迁移率族蛋白B1
Lumbricus
Pneumosilicosis
High mobility group protein B1
