摘要
[目的]通过银杏叶提取物改善矽肺大鼠肺内淋巴转运,探讨其对矽肺病程的影响。[方法]Wistar雄性大鼠随机分为正常对照组、处理对照组、染尘组及处理组,每组按不同采样时间又分为第7天、第14天、第28天、第42天、第56天5个亚组,每组6只。染尘组及处理组大鼠以非气管暴露法气管内注入1 mL二氧化硅混悬液(50 mg/mL);处理组每日经食道灌注银杏叶提取物混悬液(100 mg/kg);正常对照组灌入等剂量生理盐水,处理对照组灌入等剂量银杏叶提取物混悬液。分别检测肺组织中羟脯氨酸、血管内皮生长因子受体-3(VEGFR-3)及淋巴液中硅元素水平。[结果]染尘组和处理组与对照组相比,大鼠染尘后VEGFR-3及淋巴液硅元素水平均在时间上呈先增高后降低趋势,于第14天达高峰;羟脯氨酸含量持续增加。与染尘组比较,处理组第28~56天VEGFR-3水平升高(P<0.05),淋巴液硅元素水平在第7~28天亦升高(P<0.05);各时间点处理组羟脯氨酸含量均低于染尘组(P<0.05)。Pearson相关性分析显示,染尘组及处理组淋巴液硅元素与肺组织VEGFR-3均呈正相关(r=0.651,P<0.01;r=0.613,P<0.01);硅元素与肺组织羟脯氨酸均呈负相关(r=-0.786,P<0.01;r=-0.899,P<0.01)。[结论]银杏叶提取物可能通过促进淋巴循环,加快肺内二氧化硅的排除。
[Objective] To explore the effect of lymphatic transportation on the pathogenesis of silicosis in rats by promoting the intrapulmonary lymphatic transportation by Ginkgo biloba extract.[Methods] Wistar male rats were randomly divided into normal control, treatment control, silicosis model, and treatment groups. Each group was further divided into five subgroups according to sampling time of 7, 14, 28, 42, and 56 d, respectively, with six rats in each subgroup. The rats in the silicosis model group and the treatment group were injected with 1 mL silica suspension（50 mg/mL） by intratracheal instillation. The treatment group was injected with Ginkgo biloba extract suspension（100 mg/kg） everyday by gavage. The normal control was given saline at the same volume, and the treatment control group was given Ginkgo biloba extract suspension at the same volume. The levels of hydroxyproline（HYP） and vascular endothelial growth factor receptor-3（VEGFR-3） in lung and silicon in lymph were measured for each group.[Results] Compared with the control group, both the VEGFR-3 and silicon in lymph rose up and peaked on day 14, while the HYP level continuously increased over time in both the silicosis model group and the treatment group. Compared with the silicosismodel group, the level of VEGFR-3 in the treatment group significantly increased from day 28 to day 56（P 〈 0.05）, the level of silicon in lymph also increased from day 7 to day 28（P 〈 0.05）, and the level of HYP decreased at all time points（P 〈 0.05）. The level of silicon in lymph was positively correlated with the level of VEGRF-3 in lung in the model group and the treatment group（r=0.651, P 〈 0.01; r=0.613, P 〈 0.01）, and negatively correlated with the level of HYP（r=-0.786, P 〈 0.01; r=-0.899, P 〈 0.01）, according to the results of Pearson correlation analysis.[Conclusion] Ginkgo biloba extract could promote lymphatic circulation and accelerate lymphatic transport of silica in lung.
作者
陈紫莺
候晓敏
崔洁
田盈
马景景
郝小惠
郭灵丽
马凤丹
刘和亮
CHEN Zi-ying;HOU Xiao-min;CUI Jie;TIAN Ying;MA Jing-jing;HAO Xiao-hui;GUO Ling-li;MA Feng-dan;LIU He-liang(School of Public Health;Medical Research Center, North China University of Science and Technology, Tangshan, Hebei 063210, China)
出处
《环境与职业医学》
CAS
CSCD
北大核心
2018年第4期366-370,共5页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金项目(编号:81673119)
唐山市科技计划项目(编号:15130212C)
河北省高等学校科学技术研宄项目(编号:ZD2016026)
关键词
矽肺
淋巴循环
银杏叶提取物
硅元素
血管内皮生长因子受体-3
silicosis
lymphatic circulation
Ginkgo biloba extract
silicon
vascular endothelial growth factor receptor-3
