摘要
目的 研究骆驼奶对小鼠肠道黏膜固有层细胞的影响.方法 单次实验中将6只6～8周龄雄性C57BL/6小鼠随机分为2组,骆驼奶实验组、双蒸水（DDW）对照组.构建小鼠模型,收集肠黏膜固有层细胞（LP细胞）,使用流式细胞术对小鼠肠黏膜固有层组织中免疫细胞数量比例进行分析.酶联免疫吸附法（ELISA）测定LP细胞培养上清中IL-4、IL-10、IL-17、IFN-γ的含量.结果与双蒸水对照组相比,骆驼奶实验组小鼠肠道黏膜固有层淋巴细胞中CD4＋T细胞比例和细胞绝对数均增高,产生IFN-γ的CD4＋T细胞比例和细胞绝对数均增高,差异有统计学意义（P〈0.05）,与双蒸水对照组相比,骆驼奶组IFN-γ分泌量增加,IL-4分泌量降低,差异有统计学意义（P〈0.05）.结论骆驼奶可以促进CD4＋T细胞增殖和IFN-γ产生.提示骆驼奶对正常小鼠肠道黏膜具有调节细胞增殖分化,以及调节细胞因子分泌的免疫功能调节作用.
Objective To investigate the effects of camel milk on immune cells in lamina propria （LP） of intestinal mucosa in mice. Methods Six male C57BL/6 mice（6-8 weeks） were randomly divided into two groups as follows： camel milk treatment group and double distilled water （DDW） control group. Samples of cells in LP of intestinal mucosa were collected. Cell counts and percentages of immune cells in LP were analyzed by flow cytometry. Levels of IL-4,IL-10,IL-17 and IFN-γ in the supernatants of cell cul-ture were measured by ELISA. Results Compared with the DDW control group, the camel milk treatment group showed increased percentage and absolute number of CD4＋T cells as well as IFN-γ-secreting CD4＋T cells in LP of intestinal mucosa（P〈0.05）. Moreover,significantly enhanced expression of IFN-γ and sup-pressed secretion of IL-4 were found in the camel milk treatment group （P〈0.05）. Conclusion Camel milk can promote the proliferation of CD4＋T cells and enhance the secretion of IFN-γ,indicating that camel milk regulates the proliferation and cytokine secretion of immune cells in LP of intestinal mucosa in healthy mice.
作者
崔畅婉
于淼
吴思
王爽
吴盈盈
岳原亦
孙峥嵘
Cui Changwan;Yu Miao;Wu Si;Wang Shuang;Wu Yingying;Yue Yuanyi;Sun Zhengrong(BioBank, Shengjing Hospital of China Medical Univercity, Shenyang 110004, China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2018年第2期106-110,共5页
Chinese Journal of Microbiology and Immunology
基金
国家自然科学基金（81171581）
辽宁省教育厅课题（LFWK201710）
关键词
驼骆奶
固有层淋巴细胞
免疫功能
Camel milk
Lamina propria lymphocyte
Immunologic function
