摘要
为有效切割端粒酶逆转录酶mRNA以抑制端粒酶活性,设计合成了针对端粒酶逆转录酶mRNA的核酶基因hterl RZ及htert-5'RZ,构建了核酶基因的体外转录和真核表达质粒。将核酶转染至肿瘤细胞中,均能抑制端粒酶活性。核酶htert RZ稳定转染细胞后,使细胞倍增时间延长,但无明显细胞凋亡。因而,二种核酶可望成为有效的端粒酶抑制剂,用于抑制肿瘤生长。
Human telomerase reverse transcriptase (hTERT) is the catalytic subunit and a key factor which controls the telomerase activity, so it is the best choice to inhibit telomerase through controling hTERT expression. In research two ribozymes (htert RZ and htert 5'RZ)were designed and synthesized to serve as a telomerase inhibitor. To investigate its in vivo effect of telomerase inhibition on tumor cells, these ribzymes were tranfected by lipofectamine into tumor cells transiently or permenantly. Tumor cell with ribozyme genes clearly reduced the level of telomerase activities. All of transfectants with pcDNA3-htert RZ clearly grew more slowly than did the parental cell line. The doubling time of the tansfectants prolonged compared to the negative control, but no apparent apoptosis was showed although at their 37 population doublings. These findings support the potential application of these ribozymes as new theraputic agents directed against immortalized cancer cell.
出处
《科学技术与工程》
2002年第4期37-40,共4页
Science Technology and Engineering
基金
国家自然科学基金(39970836)
