摘要
SlMC7 prokaryotic expression recombinant protein was used as test materials. SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli. ELISA and protein dot plot methods were used to detect the antibody titer and expression characteristics and the expression difference at different stages,providing material foundation and reference basis for subsequent SlMC7 functional research in tomato fruit. The results showed that the titer of SlMC7 polyclonal antibodies was more than 1∶ 256 000 and the sensitivity of SlMC7 polyclonal antibodies was 1. 6 ng at the dilutability of 1∶ 10 000. SlMC7 protein was detected at different stages,and SlMC7 had a higher expression level in color-break fruit,indicating that SlMC7 might be relevant to the fruit ripening of tomato.
SlMC7 prokaryotic expression recombinant protein was used as test materials. SlMC7 polyclonal antiserum was obtained from immunized mice by using purified SlMC7 recombinant protein from Escherichia coli. ELISA and protein dot plot methods were used to detect the antibody titer and expression characteristics and the expression difference at different stages,providing material foundation and reference basis for subsequent SlMC7 functional research in tomato fruit. The results showed that the titer of SlMC7 polyclonal antibodies was more than 1∶ 256 000 and the sensitivity of SlMC7 polyclonal antibodies was 1. 6 ng at the dilutability of 1∶ 10 000. SlMC7 protein was detected at different stages,and SlMC7 had a higher expression level in color-break fruit,indicating that SlMC7 might be relevant to the fruit ripening of tomato.
作者
Zhichao WANG
Qiumin MA
Yanxiong YE
shuai WEN
Jingshun CAO
Guiqin QU
Zhichao WANG;Qiumin MA;Yanxiong YE;shuai WEN;Jingshun CAO;Guiqin QU(Henan Provincial Food and Drug Evaluation and Inspection Center Zhengzhou 450046;College of Food Science and Nutritional Engineering,China Agricultural University ,Beijng 100083,china)
基金
Supported by National Natural Science Foundation of China(31672207)
