摘要
旨在研究脂多糖应激对肉仔鸡生长性能、肠道微生物菌群和肉品质的影响,确定脂多糖作为免疫应激模型的最适使用剂量提供指导支持,为阐明免疫应激状态下动物生长代谢变化及从营养角度研发抗免疫应激药物奠定基础。将120只肉仔鸡随机分成4个处理组(对照组、Ⅰ组、Ⅱ组、Ⅲ组),分别在16、18、20日龄,Ⅰ组、Ⅱ组、Ⅲ组分别腹腔注射100、500、1 000μg/kg体重的脂多糖,对照组注射生理盐水,试验期为42d,通过检测平均日增重、平均日采食量、料重比、大肠埃希菌、乳酸菌、双歧杆菌、屠宰率、半净膛率、全净膛率、肉色、pH、剪切力、失水率等指标,比较分析脂多糖不同注射剂量对肉仔鸡生长性能、肠道微生物菌群和肉品质的影响。结果显示,Ⅰ组、Ⅱ组、Ⅲ组与对照组相比,平均日采食量、平均日增重、乳酸菌数、双歧杆菌数、a*值、pH均显著降低(P<0.05),大肠埃希菌数、失水率均显著升高(P<0.05);Ⅱ组、Ⅲ组与Ⅰ组相比,平均日采食量、平均日增重、双歧杆菌数、pH均显著降低(P<0.05),大肠埃希菌数、失水率均显著提高(P<0.05);Ⅲ组与Ⅱ组相比,平均日增重显著降低(P<0.05),失水率显著提高(P<0.05)。表明脂多糖应激能引起肉仔鸡肠道微生物菌群平衡发生改变,导致肉鸡生长性能、屠宰性能和肉品质下降,而且应激强度越大,负面效应越强。
To investigate the effects of lipopolysaccharide stress on growth performance,intestinal microflora and meat quality in broilers,the optimal dosage LPS as immune stress model was determined for clarif- ying the immune stress and metabolic changes in animal growth and development from the angle of nutrition to develop anti immune stress drugs.120 broilers were randomly divided into 4 groups （control group, group Ⅰ ,group Ⅱ ,group Ⅲ ）, respectively in 16,18,20 days of ages,groups Ⅰ , Ⅱ , Ⅲ were intraperitoneal injected with lipopolysaccharide 100,500,1 000 μg/kg,the control group injected with saline, test for a period of 42 d.The average daily weight gain,average daily feed intake,feed conversion ratio,Escherichia coli ,Lactobacillus,Bifidobacterium, slaughter rate, semi eviscerated rate, eviscerated rate, meat color, pH, shear force, water loss rate and other indicators were measured to analyz the effects of different doses of lipopolysaccharide on growth performance, intestinal microflora and meat quality in broilers. The results showed that compared with control group , the average daily feed intake , average daily weight gain , the number of Lactobacillus,Bifidobacterium,a ＊ and pH were significantly decreased （P〈0.05） ,Escherichia coli ,water loss rate increased significantly（P〈0.05） in groupⅠ , Ⅱ , Ⅲ compared with and group Ⅰ ,the average daily feed intake,average daily gain, the number of Bifidobacterium, pH decreased significantly（P 〈0.05）, the number of Escherichia coli and water loss rate were significantly increased（P 〈0.05） in groups Ⅱ , Ⅲ ; compared with group Ⅱ , the average daily weight gain was significantly decreased （P〈0.05） ,the water loss rate significantly improved （P〈0.05） in group Ⅲ .The lipopolysaccharide can cause intestinal microflora balance changes,resulting in a decline in broiler growth performance, slaughter performance and meat quality,and the more stress strength, the more negative effect.
作者
马邯生
林婧
马贵达
石玉祥
史瑞雅
MA Han-sheng;LIN Jing;MA Gui-da;SHI Yu-xiang;SHI Rui-ya(College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, Hebei, 056038, China;Laboratory of Conventional Pathogen Detection of Veterinary Medicine ,College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan , Hebei , 056038, China;Kay Laboratory of Veterinary Medicine of Handan City, Handan , Hebei , 056021, China)
出处
《动物医学进展》
北大核心
2018年第4期115-119,共5页
Progress In Veterinary Medicine
基金
河北省自然科学基金项目(C015402110)
关键词
脂多糖
肉鸡
生长性能
肠道微生物菌群
屠宰性能
肉品质
lipopolysaecharide
broiler
growth performance
intestinal microflora
slaughter performance
meat quality
