摘要
目的建立用氨基柱的高效液相色谱(HPLC)法测定利巴韦林片的含量。方法采用Agilent ZORBAX NH2色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(75∶25,V/V),检测波长为207 nm,柱温为30℃,进样量为20μL。结果在选定的色谱条件下,利巴韦林在12.95~103.6μg·mL^(-1)的浓度范围内与其峰面积呈良好的线性关系(r=0.999 9),平均回收率为99.9%,RSD=1.0%(n=9)。结论本法简便、快速、准确、重复性好,适用于利巴韦林片的质量控制。
OBJECTIVE To establish a HPLC method with NH2 column for the determination of the content of Ribavirin tablets.METHODS Agilent ZORBAX NH2（ 4.6 mm×250 mm,5 μm） column was used with acetonitrile and water（ 75 ∶25,V/V） as mobile phase.The detection wavelength was 207 nm.The column temperature was set at 30 ℃,and the injection volume was 20 μL. RESULTS A good linearity was shown at the concentration of 12.95-103.6 μg·mL（-1）（ r= 0.999 9） of Ribavirin,the average recovery rate was 99.9%with RSD = 1.0 %（ n = 9）. CONCLUSION This method is simple,rapid,accurate and reliable,which is suitable for the quality control of Ribavirin tablets.
作者
木其尔
赵英奎
MU Qier;ZHAO Y ingkui(Dandong Drug Inspection Test Center , Dandong , Liaoning 118002 , China)
出处
《今日药学》
CAS
2018年第4期249-251,共3页
Pharmacy Today
