摘要
目的通过研究不同浓度孔石莼多糖(U)及硫酸酯化孔石莼多糖(HU)对人肝癌细胞HepG2中内源性低密度脂蛋白受体(LDL)表达和LDLR mRNA转录的影响,探讨U和HU在受体及基因水平上的调脂作用机制。方法利用Western-Blotting技术测定U和HU对HepG2细胞LDLR表达量的影响,采用荧光定量PCR技术分析不同浓度的U和HU对人肝癌细胞HepG2LDLR mRNA表达的影响。结果 HepG2细胞经不同浓度的U和HU作用后,U和HU用药组的LDLR表达量显著高于空白对照组(P<0.05),证明从海藻孔石莼中提取的U和HU可显著提高人肝癌细胞HepG2中LDLR的表达水平。同时,HU药物组的LDLR表达量明显高于U药物组(P<0.05)。U和HU用药组其LDLR mRNA的含量与空白对照组相比均明显升高(P<0.05)。其中U药物组(0.1mg·mL-1)对HepG2细胞中LDLR mRNA的升高作用最明显,与空白对照组相比提高了约7.72倍。HU药物组(0.1mg·mL-1)对HepG2细胞中LDLR mRNA的升高作用弱于U药物组(0.1mg·mL-1),与空白对照组相比提高了约3.89倍。结论 U和HU可能通过促进HepG2细胞中LDLR mRNA,增加肝细胞LDLR蛋白表达量,从而降低LDL浓度。
Objective To study the possible mechanism of Ulva pertusa polysaccharide（U）and its sulfate derivative（HU）on decreasing blood-lipid,the effect of U and HU on the expression of receptor（LDLR）,and LDLR mRNA of the LDL in the human hepatoma cell line HepG2 in vitro were investigated.Methods Western-blotting was used to determinate the effect of U and HU on the expression of LDLR,and PCR technology was used to determinate the expression of LDLR mRNA in HepG2 cells.Results The expression of LDLR in U and HU groups was significantly higher than that in blank control group（P〈0.05）.That was to say the U and HU could obviously improve the expression of LDLR in HepG2.And the expression of LDLR in the HU group was higher than that in the U group.By the PCR technology,the expression of LDLR mRNA in the groups of U and HU was significantly higher than that in the blank control group（P〈0.05）.In the U group（0.1 mg·mL-1）,the expression of LDLR mRNA was increased about 7.72 times compared with the blank control group.And the expression of LDLR mRNA of the HU group（0.1 mg mL-1）was increased about 3.89 times than that in the blank control group.But the effect of U was stronger than HU on the expression of LDLR mRNA.Conclusion U and HU could decrease the concentration of LDL by promoting the expression of LDLR and LDLR mRNA in HepG2 cells.
作者
王凯
王中红
李芳萍
高志强
刘法荣
綦慧敏
WANG Kai;WANG Zhong-hong;LI Fang-ping;GAO Zhi-qiang;LIU Fa-rong(Weifang No. 2 People＇s Hospital, Weifang 261041, China;Affiliated Hospital of Weifang Medical University, Weifang 261041, China;Weifang Medical University, Weifang 261053, China)
出处
《中国海洋药物》
CAS
CSCD
2018年第2期81-87,共7页
Chinese Journal of Marine Drugs
基金
国家自然科学基金项目(41206129)
山东省自然科学基金项目(ZR2012DL13)
山东省医药卫生发展计划项目(2016WS0665)资助
关键词
孔石莼多糖
硫酸酯化孔石莼多糖
人肝癌细胞
低密度脂蛋白受体
Ulva pertusa polysaccharide
sulfate derivative
human liver cancer cells
low density lipoprotein receptor
