摘要
目的:探讨针刺肝俞对大鼠原位肝移植急性排斥反应的影响。方法:建立肝移植急性排斥模型,80只模型大鼠按随机数字表法分为对照组(生理盐水组)、治疗A组(针刺肝俞组)、治疗B组(他克莫司组)、治疗C组(针刺肝俞+他克莫司组),每组20只。治疗A组针刺肝俞穴,1次/d,每次20 min;治疗B组给予他克莫司0.05 mg/(kg·d)灌胃,1次/d;治疗C组给予他克莫司和针刺肝俞。观察各组大鼠生存率及生存时间,术后第7天取腔静脉血检测肝功能各项指标[总胆红素(TBi L)、结合胆红素(DBi L)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(ALP)及谷氨酰转肽酶(GGT)]。结果:与对照组相比,治疗各组的大鼠术后存活时间明显延长,肝功能各项指标均明显下降(P<0.05);与治疗A组比较,治疗B和治疗C组的大鼠术后存活时间明显延长,其肝功能指标也均显著下降(P<0.05);与治疗B组比较,治疗C组的大鼠术后存活时间明显延长,其肝功能各项指标明显下降(P<0.05)。结论:针刺肝俞能有效地减轻大鼠肝移植急性排斥反应,提高大鼠术后生存时间,其可应用于临床抗肝移植急性排斥反应治疗。
Objective： To explore the impact o f acupuncture Ganshu point on acute rejection（AR） after orthotopic liver transplantation（OLT） in rats. Methods： The model of OLT with AR was established in 80 rats, these racs were divided into four groups by random number table： control group（normal saline group）, acupuncture Ganshu point group（group A）, tacrolimus group（group B）,tacrolimus and acupuncture Ganshu group（group C）, 20 in each group.Given acupuncture Ganshu in rats of group A, one time a day, 20 minutes each time; given tacrolimus intragastric administration of 0.05 mg/（kg·d） one time a day to rats of group B; given tacrolimus intragastric administration [0.05 mg/（kg·d）, one time a day] and acupuncture Ganshu（one time a day, 20 minutes each time） in rats of group C. Analysis the survival rate of four groups, vena cava blood was taken to measure the levels of TBi L, DBi L, AST, ALT, ALP, GGT on postoperative day 7. Results： Compared with the control group, the survival time of rats treated with each group was significantly prolonged, and the indexes of liver function were significantly decreased（P0.05）. Compared with group A, the survival time of rats treated with B and C was significantly prolonged and the liver function index decreased significantly（P 0.05）. Compared with the treatment group B, the survival time of rats in group C was significantly prolonged, and the indexes of liver function were significantly decreased（P0.05）. Conclusion： Acupuncture Ganshu point can alleviate the acute rejection of liver transplantation in rats definitely, it provides a new animal experimental basis for the treatment of acute rejection.
作者
丁利民
邓丽珊
卢泳
DING Li-min;DENG Li-shan;LU Yong(The people＇s hospital of Jiangxi province, Nanehang330000;The disabled culture sports management center in Jiangxi province, Nanehang330000)
出处
《实用中西医结合临床》
2018年第1期1-2,54,共3页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
江西省卫计委中医药科研课题(编号:2015A029)
关键词
大鼠原位肝移植
针刺肝俞
急性排斥反应
生存率
肝功能
Orthotopic liver transplantation in rats
Acupuncture Ganshu
Acute rejection
Survival rate
Liver function
