摘要
目的探索纯钛表面纳米结构对细胞的生物活性,对纯钛表面改性研究具有重要意义。本实验通过制备独特的簇状锐钛矿/金红石纳米线(AR@NWs)并进一步探讨是否有利于成骨细胞的早期生物学反应。方法采用水热法制备独特的AR@NWs。主要通过三步合成过程制备,进一步通过X射线衍射仪验证纳米线表面晶相结构,并通过体外细胞实验探索纳米线的生物活性。结果本实验制备出的纳米线直径约为200 nm。制备过程阶段可以产生3种形貌相似、极具润湿性但晶相不同的纳米线,体现出不同的生物特性。细胞实验中,相对钛酸钠纳米线(STi@NWs)和钛酸纳米线(HTi@NWs),AR@NWs表面利于细胞粘附,显示出表面化学组成和晶相在细胞的早期反应中起着重要的作用,即一定程度上,锐钛矿和金红石的产生可以补偿纳米线的细胞排斥性。结论纯钛表面除了表面形貌等物理特性,表面化学特性在促进细胞早期生物活性方面也起到非常重要的作用。
Objective Exploration of the titanium surface nano structure on the cellular bioactivity is ofgreat significance to modifying the pure titanium surface. The unique cluster-like anatase/rutile nanowires（AR@NWs）was prepared and we further examined whether this surface was beneficial for early biological reactionin osteoblast. Methods Unique cluster-like anatase/rutile NWs（AR@NWs）was generated by using a simple hy-drothermal reaction via a three-step synthesis process. The crystal structure of nanowires on titanium surface was de-tected by X-ray diffractometer. The biological activity of nanowires was studied using in vitro cellular experiments.Results The AR@NWs was with a diameter of 200 nm. Three different types of NWs were generated during theproduction process,displaying different crystal structure and biological characteristics but similar surface topogra-phy and wettability. Compared with sodium titanate NWs（STi@NWs）and H2Ti2O5 nanowires（HTi@NWs）,AR@NWs surface was conductive to cell attachment. The data indicated that the surface titanium chemical composi-tion and crystal structure played an important role in the cell early response. To some extent,the generation of ana-tase and rutile compensated the cell-repelling properties of NWs. Conclusion Not only the surface physical prop-erties such as surface topography but also the surface chemistry plays an important role in promoting the cell earlybioactivity.
作者
高岩
刘影
徐淑兰
周磊
GAO Yan;LIU Ying;XU Shulan;ZHOU Lei(Center of Oral Implantology, Stomatological Hospital, Southern Medical Uni- versity, Guangzhou 510280, China)
出处
《实用医学杂志》
CAS
北大核心
2018年第8期1248-1252,共5页
The Journal of Practical Medicine
基金
广东省自然科学基金项目(编号：2015A030310071)
广东省医学科学技术研究基金项目(编号：A2016356
A2016212)
广东省科技计划项目(编号：20140212)
南方医科大学科研启动计划青年培育项目(编号：PY2017N039)
