摘要
目的探究口腔扁平苔藓(OLP)患者miR-19a与TNF-α的表达情况,分析二者与临床病理参数的关系,并分析二者的相关性。方法 2015年1月—2016年1月宁波市第二医院经病理诊断为OLP患者的口腔黏膜组织标本43例作为研究组,另取同期口腔鳞状细胞癌组织标本30例和体检健康者正常口腔组织标本30例作为对照组,应用实时荧光定量PCR(qRT-PCR)检测组织中miR-19a与TNF-αmRNA的表达情况,并分析其与口腔扁平苔藓患者临床参数的关系。结果与正常口腔黏膜组相比,口腔扁平苔藓组中miR-19a(t=5.912,P<0.001)和TNF-αmRNA(t=21.938,P<0.001)表达水平显著增加。与口腔鳞状细胞癌组相比,口腔扁平苔藓组miR-19a(t=3.980,P<0.001)和TNF-αmRNA(t=6.052,P<0.001)表达水平显著降低。miR-19a和TNF-α表达水平与患者年龄、性别、病程、临床表型有关(P<0.05)。在口腔扁平苔藓组织中随miR-19a表达水平的增加,TNF-αmRNA表达水平逐渐增加,二者呈正相关(r=0.495,P<0.05)。结论 OLP患者组织中miR-19a与TNF-αmRNA表达水平显著增加,与患者年龄、性别、病程及临床表型有关,且二者呈正相关。
Objective To investigate the expression of miR-19a and TNF-α in patients with Oral lichen planus （ OLP）, explore the relation to each other, and analyze the relationship between them and clinicopathological parameters. Meth- otis From January, 2015 to January, 2016, 43 cases of oral mucosal tissue from OLP patients （study group）, 30 cases of oral squamous cell carcinoma specimens （carcinoma gorup ） and 30 cases of normal oral tissue samples （control group ） were collected. The expression of miR-19a and TNF-α mRNA in different tissues from the three groups was detected by real-time quantitative PCR （qRT-PCR） , and the relationship between the expression of miR-19a and TNF-α mRNA and the clinical parameters of patients with oral lichen planus was analyzed. Results Compared with the control group, the expression levels of miR-19a（t = 5. 912, P 〈 0. 001 ） and TNF-α mRNA （t = 21. 938, P 〈 0. 001 ） in the study group were significantly increased. Compared with carcinoma group, the expression levels of miR-19a （t = 3. 980, P 〈 0. 001 ） and TNF-α mRNA （t = 6. 052, P 〈 0. 001 ） in the study group were significantly lower. The expression of miR-19a and TNF-α was correlated with age, sex, course of disease and clinical phenotype （P 〈 0.05 ）. TNF-α mRNA level increased with the expression level of miR-19a in oral mucosal tissue from OLP patients, there was an positive corre- lation （r = 0. 495 ,P 〈 0.05 ）. Conclusion The expression of miR-19a and TNF-α mRNA in oral mucosal tissue from OLP patients was significantly increased, which was related to age, sex, duration and clinical phenotype.
作者
王梁
吴伟
陈吉俊
张顺
李幼华
徐明
蔡雅卫
WANG Liang;WU Wei;CHEN Ji-jun;et al(Department of Stomatology, the Second Hospital of Ningbo, Ningbo, Zhefiang 315010, China)
出处
《中华全科医学》
2018年第6期899-902,共4页
Chinese Journal of General Practice
基金
浙江省中医药科技计划项目(2016ZA175)
浙江省宁波市自然科学基金(2016A610147)
