摘要
目的探讨脂肪干细胞条件培养基（adipose derived stem cell-conditioned medium,ADSC—CM）修复中波紫外线（ultraviolet irradiation B，UVB）损伤的效应。方法体外培养、获得不同衰老程度的成纤维细胞（humandermal fibroblasts，HDFs），UVB处理HDFs，造成HDFsUVB损伤，采用不同浓度ADSC—CM处理HDFs，于不同时间点取材检测不同组别HDFs的细胞增殖和衰老情况。结果ADSC-CM的浓度及处理时间分别为100%和48h最佳；对于不同衰老程度的HDFs，无论是否经过uVB处理，ADSC-CM均可较普通培养液显著提高细胞的增殖活性。结论内源性因素及外源性因素分别作用及共同作用均可引起HDFs的老化损伤，ADSC—CM可一定程度减弱HDFs的内源性、外源性老化损伤。
Objective To study the differences of UVB damage among yong, intermediate and aged human dermal fibroblasts （HDFs）, the recovery effect of adipose-derived stem cell conditioned medium （ADSC-CM） on UVB damage and the relationship between the recovery effect and ADSC-CM concentration as well as its treating period. Methods HDFs were cultured at different senescent de- grees in vitro to establish UVB damage model of HDFs, and then the HDFs were cultured in different concentration ADSC-CM and cellular proliferation was detected. Results The optimal treating period and concentration of ADSC-CM were 48h and 100% respectively. As to HDFs in different senescent degrees, ADSC-CM was able to accelerate cellular proliferation in both UVB group and control group. Conclusions Independently or simultaneously, the intrinsic and extrinsic factors can cause ageing damages on HDFs. To some extent, ADSC-CM has recovery effect on the damages through secre- tomes from ADSC.
作者
王婷
郭澍
陈鹏
曹政
张双溢
刘学晖
Wang Ting;Guo Shu;Chen Peng;Cao Zheng;Zhang Shuangyi;Liu Xuehui.(Department of Plastic Surgery, the First Hospital of China Medical University, Shenyang 110001, China)
出处
《中华医学美学美容杂志》
2018年第2期79-83,共5页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
国家自然科学基金资助项目（51272286）
辽宁省医学高峰建设工程资助项目（2010014）
辽宁省自然科学基金资助项目（20102296）
