摘要
为探寻南瓜中对正常及糖尿病模型小鼠血糖有影响的未知有效成分 ,本实验依次采用有机溶剂分步萃取、除蛋白、透析、乙醇沉淀及葡聚糖柱层析等分离手段 ,以小白鼠血糖值作为筛选活性成分的指标 ,在分离的每一阶段对分离所得各个组分进行活性定量评估 ,并追踪活性最强的部分 ,最后分离纯化出降血糖活性最强的组分G———南瓜多糖。高效液相色谱证明其为杂多糖 ,分子量为 1.16× 10 5。
This experiment adopted organic solvent extraction step by step,following deproteinization,dialyzation,alcohol-precipitation and layer-separation of dextran,to reveal the unknown effective ingredients in pumpkin,which have influence to blood sugar in normal mice or model mice of diabetes mellitus.The most active group-elements G for reducing blood sugar was pumpkin polysaccharide.It was proved by HPLC that the element G was a heterogeneous polysaccharide and its molecular weight was 1.16×10 5.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2002年第4期59-62,共4页
Journal of the Chinese Cereals and Oils Association
