摘要
目的·研究系统性红斑狼疮(systemic lupus erythematosus,SLE)患者CD4^+CD25^+CD45RA^-T细胞中转录因子BTB与CNC同源基因2(BTB and CNC homology 2,Bach2)的表达及其对细胞功能的影响。方法·采用流式细胞仪分选获得活动期SLE患者(SLE组)和健康对照者(对照组)外周血CD4^+CD25^+CD45RA^-T细胞,荧光定量PCR和Western blotting检测该类细胞中Bach2的表达;分析SLE患者该类细胞中Bach2的平均荧光强度(median fluorescence intensity,MFI)与SLE疾病活动指数(systemic lupus erythematosus disease activity index,SLEDAI)的相关性。采用流式细胞术比较SLE患者IL-17+CD4^+CD25^+CD45RA^-T细胞和IL-17-CD4^+CD25^+CD45RA^-T细胞中Bach2的MFI。在Bach2过表达体系中,采用流式细胞术检测CD4^+CD25^+CD45RA^-T细胞表达IL-17的细胞比例,采用ELISA方法检测细胞培养上清液中IL-17的含量。结果·与对照组相比,SLE组CD4^+CD25^+CD45RA^-T细胞Bach2的m RNA和蛋白表达水平均显著降低(均P<0.01);SLE组Bach2的MFI与SLEDAI呈显著负相关(R2=0.433,P=0.001)。SLE患者IL-17+CD4^+CD25^+CD45RA^-T细胞Bach2的MFI显著低于IL-17-CD4^+CD25^+CD45RA^-T细胞(P=0.013)。当Bach2过表达时,SLE患者CD4^+CD25^+CD45RA^-T细胞表达炎症因子IL-17的细胞比例明显下降(P=0.032),细胞培养上清液中IL-17含量显著降低(P=0.008)。结论·SLE患者CD4^+CD25^+CD45RA^-T细胞Bach2表达下降,在该类细胞中过表达Bach2可使IL-17表达下降。
Objective · To study the expression of transcription factor BTB and CNC homology 2（Bach2） in CD4~＋CD25~＋CD45RA^-T cells from patients with systemic lupus erythematosus（SLE） and its effect on cell function. Methods · The CD4~＋CD25~＋CD45RA^-T cells from active SLE patients and healthy volunteers were sorted by flow cytometry. The expression of Bach2 in CD4~＋CD25~＋CD45RA^-T cells was detected by fluorescence quantitative PCR and Western blotting. The correlation between the median flourscence indensity（MFI） of Bach2 in CD4~＋CD25~＋CD45RA^-T cells and the disease activity index of SLE（SLEDAI） was analyzed. The MFI of Bach2 in IL-17＋CD4~＋CD25~＋CD45RA^-T cells was compared with that in IL-17-CD4~＋CD25~＋CD45RA^-T cells by flow cytometry. In Bach2 overexpression system, the expression of IL-17 in CD4~＋CD25~＋CD45RA^-T cells was detected by flow cytometry and the concentration of IL-17 in the culture supernants was detected by ELISA. Results · The m RNA and protein expressions of Bach2 in CD4~＋CD25~＋CD45RA^-T cells from SLE patients were significantly lower than those in healthy controls（P0.01）. There was a significant negative correlation between the MFI of Bach2 and SLEDAI（R2=0.433, P=0.001） in patients with SLE. The expression of Bach2 in IL-17＋CD4~＋CD25~＋CD45RA^-T cells was significantly lower than that in IL-17-CD4~＋CD25~＋CD45RA^-T cells（P=0.013）. When Bach2 was overexpressed, the percentage of CD4~＋CD25~＋CD45RA^-T cells from SLE patients expressing inflammatory factor IL-17 decreased significantly（P=0.032） and the IL-17 concentration in cell culture supernatants markedly decreased（P=0.008）. Conclusion · The expression of Bach2 in CD4~＋CD25~＋CD45RA^-T cells from SLE patients decreases, and overexpression of Bach2 in the cells leads to the falling expression of IL-17.
作者
陆一闻
郑英霞
潘秀军
聂红
LU Yi-wen1, 2, ZHENG Ying-xia2, PAN Xiu-jun2, NIE Hong1(1. Department of Immunology and Microbiology, Shanghai Jiao Tong University College of Basic Medical Sciences, Shanghai 200025; 2. Department of Laboratory Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 20009)
出处
《上海交通大学学报（医学版）》
CAS
CSCD
北大核心
2018年第4期369-373,共5页
Journal of Shanghai Jiao tong University:Medical Science
基金
国家自然科学基金面上项目(81571525
81671590)~~
