摘要
采用硅胶柱层析法对臭蒿粗提物进行分离纯化,利用孢子萌发法对抗菌流分进行活性评价,采用高效液相色谱-质谱联用技术(HPLC-MS)分析抗菌流分的物质组成。结果显示:臭蒿粗提物分离纯化得到13种流分(Fr.1~Fr.13),其中流分Fr.5抗菌活性最好,对流分Fr.5进一步硅胶柱层析分离得到4种流分(Fr.5.1~Fr.5.4)。臭蒿粗提物对烟草链格孢菌孢子的EC50为5.04 g/L,流分Fr.5.2的EC50为0.57 g/L。LC-MS分析出流分Fr.5.2主要由去氧双羟基蒿甲醚(17.00%)、去氧双氢青蒿素(43.65%)、去氧青蒿素(16.15%)组成。以双氢青蒿素为标准品,测得流分Fr.5.2中双氢青蒿素的质量分数为40.95%,双氢青蒿素对烟草链格孢菌孢子的EC50为2.70 g/L,表明流分Fr.5.2中主要抗菌物质为青蒿素衍生物。
The extraction of Artemisia hedinii Ostenf. et Pauls（ A. hedinii） were rough and further isolated by chromatographic separation technology, and the antifungal activity of the component was evaluated by the the spore germination method.Furthermore,the composition of antimicrobial components was analyzed by high performance liquid chromatography-mass spectrometry（ HPLC-MS）. The results showed that 13 kinds of components（ Fr.1-Fr.13） were rough isolated from the extraction of A. hedinii,in which the component Fr.5 showed the best antifungal activity. Four kinds of components（ Fr.5.1-Fr.5.4） were obtained by the further separation of the component Fr. 5. The EC50 of A. hedinii crude extraction on Alternaria alternate（ A.alternate） spores was 5.04 g/L,and that of the component Fr.5.2 was 0.57 g/L. The HPLC-MS results showed that the components of Fr.5.2 mainly contained deoxy double hydroxyl artemether（ 17 %）,deoxy dihydroartemisinin（ 43. 65 %） and deoxyartemisinin（ 16. 15 %）. The content of dihydroartemisinin of component Fr. 5. 2 was calculated as 40. 95 % by using dihydroartemisinin as the standard. The EC50 of dihydroartemisinin on A. alternate was 2. 70 g/L,indicating that the major antifungal matter in component Fr.5.2 was artemisinin derivatives.
作者
徐杰
程传可
胡国元
杨春雷
余君
杨锦鹏
XU Jie;CHENG Chuanke;HU Guoyuan;YANG Chunlei;YU Jun;YANG Jinpeng(School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology-, Wuhan 430205, China;Tobacco Research Institute of Hubei Province, Wuhan 430030, China)
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2018年第2期80-86,共7页
Chemistry and Industry of Forest Products
基金
中国烟草总公司重点科技项目(中烟办【2013】212号)
关键词
臭蒿
烟草链格孢菌
毒力
青蒿素衍生物
Artemisia hedinii
Alternaria alternate
toxicity
artemisinin derivatives
