摘要
目的调查样品中常见成份对"咖啡环"法DNA检测的影响。方法将含有不同浓度SDS、Na Cl等的DNA-EB滴加到载玻片上,室温自然蒸发,用凝胶成像系统观察荧光沉积形貌(Deposition Pattern,DP)、计算积分光密度(Integrated Optical Density,IOD),分析量效关系。结果低浓度非DNA成份存在时,DP仍以环状沉积为主,但有年轮环、放射纹等成份特异性细微差异。浓度高时,DP因成份而异,可为环+散在光点型(Na Cl)、中央光斑+弱小环型(SDS)、同心或年轮状多环型(Triton X-100)、环+斑点型(H^+)等。非DNA成份对DNA含量估计有不同程度影响,但估计值多数在0.5~2倍以内。结论样品成份对DNA的DP和IOD均有影响,但不影响DNA含量的粗略估计,且能提供样品纯度和残留物信息。
Objective To investigate the influences of drop components on the＇coffee ring＂ test of DNA. Methods The DNA-EB drops containing SDS, NaCl, etc, were evaporated on glass slides. After evaporation, the fluorescent Deposition Pattern （DP） and Integrated Optical Density （IOD） was acquired with a gel imaging system. The dose-effect relationship was analyzed. Result When the non-DNA components concentration is low, the DP was still characteristiced by peripheral rings, subtle component-specific differences, such as tree-ring like structure and radial crack, existed. At high concentrations, DP was various, which may be ring ＋ scattered dots （NaCl）, central spot ＋ small weak ring （SDS）, concentrc/tree-fing （TritonX-100） or ring ＋ spot （H＋）, et al. Non-DNA components had little effect on IOD（0.5-2 times）. Conclusion Non-DNA components in DNA-EB drops influences both the DP and IOD, but rough estimation of DNA concentrations is still effective. Moreover, analyzing the DP can provide more informations about sample purity and residue.
作者
詹飞
赵贵森
李亚琴
陈炯
郭琬
Zhan Fei;Zhao Guisen;Li Yaqin;Chen Jiong;Guo Wan(School of Forensic Medicine, Henan University of Science and Technology, LuoYang 471000, China)
出处
《中国法医学杂志》
CSCD
2018年第2期134-138,共5页
Chinese Journal of Forensic Medicine
基金
国家自然科学基金(81501634)
