摘要
目的:用正交试验法优选益肾涤浊丸的提取工艺。方法:采用L9(34)正交试验法,分别对药材水提和药渣醇提,考察料液比、浸泡时间、提取时间、提取次数、乙醇浓度等因素对盐酸小檗碱和干膏得率的影响。结果:水提的最佳工艺为料液比1∶10,浸泡时间6 0 min,提取时间1.5 h,提取3次;醇提的最佳工艺为料液比1∶10,乙醇浓度50%,提取时间2 h,提取3次。结论:本法提取工艺合理可行,可用于益肾涤浊丸的提取。
Objective： To optimize the extraction process of Yishen Dizhuo Pills by orthogonal design test. Methods：The effect of solid-liquid ratio,soak and extract time,extracting times,ethanol concentration on the yield of extract and the content of berberine hydrochloride extraction process of Yishen Dizhuo Pills was investigated,and the extraction process was optimized by L9（34）orthogonal test. Results： The optimized process on water-extract： solid-liquid ratio was 1∶10,soak period was 60 minute,extraction time was 1. 5 hour,extraction times was 3. The optimized process on ethanol extraction： solid-liquid ratio was 1∶10,ethanol concentration was 50%,the extraction time was 2 hour,and extraction times was 3. Conclusion： The optimized process is feasible,rational and the extraction rate of active components is optimal,which can be applied to the Yishen Dizhuo Pills extraction process.
作者
彭爱娜
杨珊
田素英
PENG Ai-na;YANG Shan;TIAN Su-ying(Guangdong Zhaoqing Hospital of Traditional Chinese Medicine, Guangdong Zhaoqing 526060, China;Guangdong Pharmaceutical University, Guangdong Guangzhou 510006, China;Guangdong Cosmetics Engineering＆Technology Research Center, Guangdong Zhongshan 528458, China)
出处
《中国现代中药》
CAS
2018年第5期609-612,共4页
Modern Chinese Medicine
基金
益肾涤浊丸的制备工艺研究(2016040304)
关键词
益肾涤浊丸
正交试验
水提药材
醇提药渣
提取工艺
Yishen Dizhuo Pill
orthogonal design test
water extraction
ethanol extraction
extraction process
