摘要
目的了解巴尔通体在广东省鼠形动物中的流行分布情况及其基因型特征,为巴尔通体的研究和自然疫源性疾病的防控提供科学依据。方法在广东省惠来县、惠东县、潮安县、罗定县和高州市5个地区进行捕鼠,采集鼠肝脏标本提取总核酸,利用聚合酶连反应(PCR)检测巴尔通体病原,并对其进行分离培养,阳性产物进行测序,并根据序列进行系统进化分析,统计分析巴尔通体的分布情况和特征。结果在广东省5个地区共捕鼠375只,检出巴尔通体阳性标本73份,阳性率为19.47%。不同鼠种(χ~2=6.361)、不同地区(χ~2=7.778)、不同性别(χ~2=0.292)、不同生境间(χ~2=0.621),阳性率差异均没有统计学意义(P>0.05)。73份样本中,10份成功分离培养出巴尔通体。系统进化分析表明广东鼠形动物携带的巴尔通体存在6种基因型:Bartonella elizabethae、B.phoceensis、B.japonica、B.henselae、B.rochalimae、B.tribocorum。结论巴尔通体在广东省鼠形动物中广泛存在,且基因型呈多样性,其中4种基因型对人类有致病性,对人类健康具有潜在威胁。
We investigated the distribution and genotype characteristics of Bartonellain rodents in Guangdong and provide scientific data for study of Bartonella and natural focal diseases control and prevention.Rodents were captured in five districts of Guangdong：Huilai,Huidong,Chaoan,Luoding and Gaozhou.Total nucleic acid from liver specimens was extracted and detected Bartonella using PCR.Positive simples were cultivated and the PCR products were sequenced.The phylogenetic analysis and statistical analysis were conducted.Results showed that totally 375 rodents were captured in five regions of Guangdong and 73 liver samples were positive for Batronella,the positive rate was 19.47%.There was no significant statistic differences between species（χ^2=6.361）,districts（χ^2=7.778）,gender（χ^2=0.292）and habitats（χ^2=0.621）（P〉0.05）.Of 73 specimens,10 were positive in Bartonellaculturing.The phylogenetic analysis illustrated that 6 genotypes of Bartonella were confirmed in rodents：Bartonella elizabethae,B.phoceensis,B.japonica,B.henselae,B.rochalimae,B.tribocorum.In Guangdong Province,Bartonellais widespread in rodents with obvious diverse genotypes.Four genotypes have potential hazard for human health.
作者
李雪云
陈经雕
黎聪荣
柯碧霞
林文青
彭志强
柯昌文
LI Xue-yun;CHEN Jing-diao;LI Cong-rong;KE Bi-xia;LIN Wen-qing;PENG Zhi-qiang;KE Chang-wen(School of Public Health,Southern Medical University,Guangzhou 510515, China;Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2018年第5期482-486,491,共6页
Chinese Journal of Zoonoses
基金
国家科技部重点专项(No.2016YFC1200201)~~
