摘要
目的:观察运脾颗粒对肝郁脾虚型功能性消化不良(Functional dyspepsia,FD)大鼠胃肠动力及胃肠激素表达的影响,探讨其防治FD的作用机理。方法:采用随机数字表法将60只SD大鼠分为空白组、模型组、运脾颗粒低、中、高剂量组及多潘立酮组,每组10只,用适度夹尾刺激为主的复合因素复制肝郁脾虚型FD大鼠模型。记录实验过程中各组大鼠的宏观表征,测定各组大鼠胃排空率及小肠推进率,RT-PCR法检测各组大鼠胃肠组织中P物质(SP)、血管活性肠肽(VIP)mRNA的表达水平。结果:与空白组比较,模型组大鼠胃排空率及小肠推进率均有不同程度的下降,结肠组织SP mRNA的表达水平下调,胃窦组织VIP mRNA的表达水平上调,差异均有统计学意义(P<0.01)。与模型组比较,运脾颗粒各剂量组及多潘立酮组大鼠胃排空率及小肠推进率显著升高,结肠组织SP mRNA表达水平上调,胃窦组织VIP mRNA表达水平下调;运脾颗粒中、高剂量组大鼠胃窦组织SP mRNA的表达水平上调,结肠组织VIP mRNA的表达水平下调,差异均有统计学意义(P<0.05,P<0.01)。与多潘立酮组比较,运脾颗粒中、高剂量组大鼠胃窦及结肠组织SP mRNA表达水平上调,VIP mRNA的表达水平下调,差异均有统计学意义(P<0.05)。结论:运脾颗粒通过对FD大鼠胃肠激素SP、VIP mRNA水平的多靶点双向调控,促进FD大鼠胃排空及肠推进功能,进一步改善FD大鼠的胃肠运动及感觉异常,是运脾颗粒防治FD的作用机理之一。
Objective： To observe the effect of Yunpi granules on gastrointestinal motility and the expression of gastrointestinal hormone of rats with functional dyspepsia（FD） of liver-depression and spleen-deficiency type,and to discuss its functioning mechanism of preventing and treating FD. Methods： Divided 60 SD rats into the blank group, the model group,the low-dose,medium-dose and high-dose Yunpi granules groups and the domperidone group randomly,10 rats in each group,and applied combined factors mainly with moderate tail-clamping stimulation to establish the model of FD rats of liver-depression and spleen-deficiency type. Recorded the macroscopic token of rats in each group during experiment,measured the gastric emptying rate and the intestinal propulsive rate of rats in each group,and detected the expression level of substance P（SP） and vasoactive intestinal peptide（VIP） mRNA in gastrointestinal tissue of rats in each group by means of RT-PCR. Results：Comparing with the blank group,the gastric emptying rate and the intestinal propulsive rate of rats in the model group were both decreased to different degrees,the expression levels of SP mRNA in colon tissue were decreased,and the expression levels of VIP mRNA in gastric antrum tissue were increased, differences being significant（P〈0.01）.Comparing with the model group,the gastric emptying rate and the intestinal propulsive rate of rats in the groups of Yunpi granules in each dose and the domperidone group were significantly increased,the expression levels of SP mRNA in colon tissue were increased, and the expression levels of VIP mRNA in gastric antrum tissue were decreased; the expression levels of SP mRNA in gastric antrum tissue of rats in the medium-dose and high-dose Yunpi granules group were increased,and the expression levels of VIP mRNA in colon tissue were decreased,differences being significant（P〈0.05,P〈0.01）.Comparing with the domperidone group,the expression levels of SP mRNA in colon and gastric antrum tissue of rats in the medium-dose and high-dose spleen-activating granules group were increased,and the expression levels of VIP mRNA were decreased, differences being significant（P〈0.05）. Conclusion： By multi-target birectional regulation of the levels of gastrointestinal hormone SP and VIP mRNA in FD rats,Yunpi granules promote gastric emptying and intestinal propulsive function of FD rats,and further improve gastrointestinal motility and paresthesia of FD rats,which is one of the functioning mechanisms of Yunpi granules for preventing and treating FD.
作者
马国珍
宋瑞平
舒劲
张晓明
王煜
卢雨蓓
MA Guozhen,SONG Ruiping,SHU Jin,ZHANG Xiaoming,WANG Yu,LU Yubei
出处
《新中医》
CAS
2018年第5期5-10,共6页
New Chinese Medicine
基金
甘肃省中医药管理局科研课题(GZK-2014-63)
关键词
功能性消化不良(FD)
运脾颗粒
肝郁脾虚型
胃肠激素
胃肠运动
动物实验
SD大鼠
Functional dyspepsia（FD）
Yunpi granules
Liver-depression and spleen-deficiency type
Gastrointestinal hormone
Gastrointestinal motility
Animal experiment
SD rats
