摘要
目的探讨胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-rP1)对鼻咽癌细胞系CNE1细胞的增殖、侵袭、迁移能力的影响。方法构建2条特异性针对IGFBP-rP1基因的小干扰RNA(siRNA32、siRNA34)及其阴性对照siRNA,将CNE1细胞分为4个转染组:siRNA32组(siRNA32转染)、siRNA34组(siRNA34转染)、阴性对照组(阴性对照siRNA转染)、空白组(不做任何处理),分别培养24h或48h后检测相关指标。结果siRNA32组和siRNA34组IGFBP-rP1mRNA及蛋白表达水平显著低于阴性对照组和空白组(P<0.05);siRNA32组和siRNA34组CNE1细胞迁移数、穿过PVP-F膜的CNE1细胞数显著低于阴性对照组和空白组(P<0.05);培养24、48h后,siRNA32组和siRNA34组CNE1细胞增殖OD值显著低于阴性对照组和空白组(P<0.05);培养48h后,siRNA32组和siRNA34组G0/G1期、G2/M期细胞所占比例显著高于阴性对照组和空白组(P<0.05);siRNA32组和siRNA34组S期细胞所占比例显著低于阴性对照组和空白组(P<0.05)。结论抑制IGFBP-rP1表达能降低鼻咽癌CNE1细胞的增殖、侵袭、迁移能力,为临床治疗鼻咽癌提供一种新的思路。
Objective To investigate the inhibitory effect of insulin-like growth factor binding protein related protein 1（IGFBP-rP1）on proliferation,invasion and migration of nasopharyngeal carcinoma cell line CNE1.Methods The construction of two small interfering RNA specific for IGFBP-rP1 gene（siRNA32,siRNA34）and negative control siRNA,CNE1 cells were divided into four groups：siRNA32 group（transfected with siRNA32 transfection）,siRNA34 group（transfected with siRNA34）,negative control group（negative control siRNA transfection）and blank group（no treatment）.After training 24 hor 48h,the related indexes were detected.Results The expression of IGFBP-rP1 mRNA and protein in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group（P〈0.05）.The number of CNE1 cells in siRNA32 group and siRNA34 group was higher than that in PVE-F membrane（P〈0.05）.The ODvalue of CNE1 cells in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group（P〈0.05）.The OD value of CNE1 cells in siRNA32 group and siRNA34 group was significantly lower than that in negative control group and blank group（P〈0.05）.The percentage of G0/G1 phase and G2/M phase cells in siRNA32 group and siRNA34 group was significantly higher than that in negative control group and blank group（P〈0.05）was lower than the negative control group and the blank group（P〈0.05）.Conclusion Inhibition of IGFBP-rP1 expression can reduce the proliferation,invasion and migration of nasopharyngeal carcinoma CNE1 cells,and provide a new idea for the clinical treatment of nasopharyngeal carcinoma.
作者
郑富春
祁晓倩
ZHENG Fuchun;QI Xiaoqian(Department of Otorhinolaryngologym;Department of Clinical Laboratory, the People＇s Hospital of Ankang ,Ankang, Shangxi 725000, China)
出处
《检验医学与临床》
CAS
2018年第10期1434-1437,共4页
Laboratory Medicine and Clinic
