摘要
以福贡龙竹种子为外植体,开展组培快繁技术研究。结果表明,用75%酒精消毒60s,再用0.1%的Hg Cl2溶液消毒30min的效果最好;适宜诱导形成丛生芽的培养基为MS+6-BA 2mg/L+IBA 0.5mg/L+KT 0.2mg/L;最佳继代增殖培养基为MS+6-BA 3mg/L+NAA 0.2mg/L;以MS+IBA 1mg/L+NAA 0.5mg/L为最佳生根培养基;将生根组培苗炼苗移栽,90d后成活率达81%以上。
The tissue culture for Dendrocalams fugongensis was conducted using its seeds as explants. The results showed that disinfection for 60 s with 75% alcohol and disinfection for 30 min with 0. 1% Hg Cl2 were most effective approach. The optimal multiple shoots inducing medium was MS ＋ 6-BA 2 mg/L ＋ IBA 0. 5 mg/L ＋ KT 0. 2 mg/L.The optimal shoots multiplication culture medium was MS＋6-BA 3 mg/L ＋NAA 0. 2 mg/L. The optimal rooting culture medium was MS＋IBA 1 mg/L ＋NAA 0. 5 mg/L. After 90 days transplanted into greenhouse,the survival rate of young plants were over 81%.
作者
呼延丽
毕玮
李灿雯
李丹
王娟
王毅
王四海
杨宇明
HU Yan-li;BI Wei;LI Can-wen;LI Dan;WANG Juan;WANGYi;WANG Si-hai;YANG Y u -ming(Yunnan Academy of Forestry,Kunming Yunnan 650201,P.R.China;Yunnan Laboratory for Conservation of Rare,Endangered ＆ Endemic Forest Plants,Public Key Laboratory of the State Forestry Administration;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants,Kunming Yunnan 650201,P.R.Chin)
出处
《西部林业科学》
CAS
北大核心
2018年第3期118-122,共5页
Journal of West China Forestry Science
基金
云南省森林植物培育与开发利用重点实验室开放基金项目(ZZCX2016-03)
云南省应用基础研究计划重点项目(2013FA054)
云南省野生动植物保护项目(2017SX1012)
关键词
福贡龙竹
组织培养
丛芽诱导
快速繁殖
Dendrocalams fugongensis
tissue cu ltu re
multiple shoots induce
rapid propagation
