摘要
目的探讨p53、p21在燃煤型氟中毒雌鼠卵巢早衰中的表达变化。方法选用断乳24只清洁级SD雌鼠建立动物模型(模型组),于染氟90d将雌鼠处死。观察雌鼠染氟期间牙齿的变化,观察卵巢颗粒细胞改变检测雌鼠尿氟、骨氟水平和卵巢组织衰老基因p53和p21表达情况。结果模型组氟斑牙、尿氟和骨氟水平明显升高。模型组中,低氟组卵巢组织颗粒细胞未见早衰,但随染氟剂量的增加,卵巢组织颗粒细胞呈现轻度、中度、重度性水肿的趋势,细胞形态损伤模糊,且闭锁的卵泡明显增加,黄体退化严重,成熟卵泡显著减少,卵巢功能呈现逐渐早衰的迹象。随染氟剂量增加,各染氟组衰老基因p53和p21表达逐渐增加,差异有统计学意义(P<0.05)。结论燃煤型氟中毒致卵巢早衰与基因p53和p21的表达明显相关。
Objective To investigate the expression changes of p53 and p21 in premature ovarian failure in female rats exposed to coal burning fluorosis. Methods Abtactation 24 SD female rats were chosen to establish animal model （the model group）,in fluorine 90 days were put to death. Observed the changes of the teeth of the female rats during fluorine exposure and ovarian granulosa cell premature aging change, detected fluorine contents of urine and bone,and expression of the ovarian granular cell gene p53 and p21. Results In the model group, fluorine spot tooth, the fluorine contents of urine and bone increased significantly. In the model group,there was no premature aging of ovarian granulosa cells in the low fluorine group, but with the increase of dye fluorine metering, ovarian granulosa cells became mild, moderate to severe progressive edema trends, cells morphological damaged fuzzy,and atresia follicles increased significantly, corpus luteum degraded, mature follicle significantly reduced, and the ovarian function gradually signs of premature aging presented. With the increase of dye fluoride measurement,the expression of aging gene p53 and p21 in each group gradually increased, compared with the control group,there was statistically significant difference （P〈0.05）. Conclusion Premature ovarian failure caused by coal-burning fluorosis was significantly related to the expression of genes p53 and p21.
作者
刘洋
李艳菊
王宁
刘振华
杨华
刘燕青
王飞清
LIU Yang1 ,LI Yanju2 ,WANG Ning3 ,LIU Zhenhua4 ,YANG Hua1,LIU Yanqing1 ,WANG Feiqing1(1. Department of Clinical Laboratory, the First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Gui yang , Guizhou 550001, China; 2. Department of Hematology, the Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, China ; 3. Department of pharmacy ,Guizhou Orthopedics Hospital ,Guiyang ,Guizhou 550002, China ; 4. Department of Hepatobiliary Surgery ,Guizhou Provincial Peoplefs Hospital, Guiyang , Guizhou 550002, Chin)
出处
《重庆医学》
CAS
2018年第13期1712-1715,共4页
Chongqing medicine
基金
贵州省科技厅项目(黔科合LH字[2017]7127
7128号)
贵阳中医学院基金项目([2017]14号)
