摘要
目的检测口腔扁平苔藓(oral lichen planus,OLP)黏膜及正常口腔黏膜中趋化因子CCL20(CC-chemokine ligand 20,CCL20)及其受体CCR6(CC-chemokine receptor 6,CCR6)的表达,研究CCR6-CCL20轴在口腔扁平苔藓病中的作用。方法通过免疫组织化学及荧光定量PCR分别检测口腔扁平苔藓病损组织及正常口腔组织中CCR6和CCL20的蛋白及mRNA的表达。结果 CCR6及CCL20在口腔扁平苔藓患者的黏膜组织中的表达均明显高于正常人,且具有统计学差异(P<0.000 1)。结论 CCR6-CCL20轴参与了口腔扁平苔藓的发生和发展,促进黏膜病损的产生及炎症细胞的浸润。
Objective To detect variation of CCR6-CCL20 expression in tissue from OLP patients and healthy controls. Methods Real-time quantitative PCR was used to detect the mRNA expression of CCR6 and CCL20 in oral mucosal tissue and immunohistochemistry was used for the detection and localization of CCR6 and CCL20. Results Tissue from OLP patients had markedly up-regulated levels of CCR6 and CCL20 mRNA compared to healthy tissue（ P 〈0.000 1）. Moreover,immunohistochemical staining showed that in OLP patients,CCR6 and CCL20 expressions were obviously higher than those in the control patients. Conclusion The CCR6-CCL20 axis may play a role in the immunological mechanism of OLP,promoting the occurrence of mucosal lesions and the immersion of inflammatory cells.
作者
李姗
朱丹丹
范媛
LI Shah, ZHU Dandan, FAN Yuan.(Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University; Department of Oral Medicine, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, Chin)
出处
《口腔医学》
CAS
2018年第5期406-409,共4页
Stomatology
基金
国家自然科学基金(81470748)
