摘要
目的研究正常恒河猴感染SIVmac239前后外周血单核细胞以及各亚群单核细胞表面CD169分子表达量的变化及可能的原因。方法正常恒河猴在静脉攻毒后,流式细胞术检测感染前后外周血单核细胞比例及其表面分子CD169表达量的变化;SIVmac239直接感染和不同细胞因子刺激流式分选出的正常恒河猴外周血CD14+单核细胞,流式细胞术检测细胞表面CD169和细胞因子IFN-α表达量的变化。结果 SIVmac239感染正常恒河猴后,CD14+单核细胞的比例下降,CD14+单核细胞表面分子CD169表达量升高;外周血中不同单核细胞亚群CD169的表达量均显著增加,CD14+CD16++单核细胞中CD169表达量的升高更为明显。正常恒河猴外周血CD14+单核细胞经细胞因子M-CSF、IL-4和IL-13刺激后,细胞表面不表达CD169;经细胞因子IFN-α刺激后,高表达CD169;SIVmac239病毒直接感染CD14+单核细胞,细胞表面CD169与胞内细胞因子IFN-α的表达均无变化。结论 SIVmac239病毒感染恒河猴后,可引起外周血单核细胞CD169分子表达量的升高,其表达与病毒直接感染单核细胞无关,与体内其它细胞分泌的细胞因子IFN-α相关。
Objective To investigate the changes of CD169 expression on the surface of peripheral blood monocytes and different subsets of monocytes in normal rhesus monkeys after SIVmac239 infection and the possible reasons.Methods Normal rhesus monkeys were infected with SIVmac239 through intravenous injection,and changes in the percentage of peripheral blood monocytes and the expression of CD169 before and after SIVmac239 infection were detected by flow cytometry. The peripheral blood CD14^＋monocytes of normal rhesus monkeys sorted by flow cytometry were directly infected by SIVmac239 and stimulated by different cytokines,and changes in the expression of CD169 on the cell surface and the cytokine IFN-α were detected by flow cytometry. Results After SIVmac239 infection,the percentage of CD14^＋monocytes of the normal rhesus monkeys was decreased and the expression of CD169 on their surface was increased.Meanwhile,the expression of CD169 on the surface of different subsets of peripheral blood monocytes was significantly increased,and the expression of CD169 on the CD14^＋CD16^＋ ^＋monocytes was increased more obviously. CD169 was not expressed on the surface of peripheral blood CD14^＋monocytes of the normal rhesus monkeys after stimulated by the cytokines M-CSF,IL-4 and IL-13. However,CD169 was highly expressed after the monocytes were stimulated by the cytokine IFN-α. The expression of CD169 on the surface of CD14^＋monocytes and the intracellular cytokine IFN-α was not significantly changed after the monocytes were directly infected with SIVmac239. Conclusions SIVmac239 infection can lead to the increase of CD169 expression on the surface of peripheral blood monocytes in rhesus monkeys. Its expression is not associated with the direct infection of virus,but is related to the cytokine IFN-α secreted by other cells of the monkeys in vivo.
作者
李想
薛婧
陈霆
丛喆
魏强
LI Xiang;XUE Jing;CHEN Ting;CONG Zhe;WEI Qiang(Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences（CAMS;Comparative MedicineCenter,Peking Union Medical College（PUMC;Key Laboratory of Human Disease Comparative Medicine,NationalHealth and Family Planning Commission of the People’s Republic of China（NHFPC;Key Laboratory of HumanDiseases Animal Model,State Administration of Traditional Chinese Medicin;Beijing Key Laboratory for AnimalModels of Emerging and Remerging Infectious Diseases,Beijing 100021,Chin)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第5期75-80,共6页
Chinese Journal of Comparative Medicine
基金
中国医学科学院医学与健康科技创新工程重大协同创新项目(编号：2017-I2M-1-014)
科技部重大专项(编号：2017ZX10304402)
