摘要
接触性过敏性皮炎是由外源化合物引起的多种细胞共同参与的IV型迟发变态反应,传统动物实验选用豚鼠或小鼠。随着皮肤致敏有害结局通路(AOP)研究的深入,为基于分子起始事件和关键事件的替代方法开发提供了思路。树突状细胞(DC)激活在AOP通路中起到了承上启下的关键作用,基于DC开发了多项替代方法,有的方法经过验证已被列入测试指南。本文从树突细胞的筛选,检测参数的特征,方法的局限性和适用性进行了讨论,并对树突细胞与其它细胞之间相互作用、共培养体系、人体芯片等方面的进展作了介绍,为进一步优化致敏检测的体外替代方法提供参考。
Allergy contact dermatitis is a type IV delayed type hypersensitivity that is induced by exogenous compounds and involves many cell types. Traditional animal testing uses guinea pigs or mice as a model. With progress of the adverse outcome pathway（AOP） on skin sensitization,a concept for development of alternative methods based on a molecular initiating event and key events is provided. Dendritic cell（DC） activation plays a key role in the AOP. Many alternative methods have been developed,with several methods validated and accepted as guidance for assays. This paper examines DC screening,characteristics of test parameters,and limitations and applicability of DC-derived methods.Progress on interactions between DCs and other cells,co-culture systems,and the human body-on-a-chip will also be introduced. Altogether,this paper will provide information for optimization of in vitro alternative methods for sensitization detection.
作者
陈健
柯逸晖
陈彧
谈伟君
程树军
CHEN Jian;KE Yihui;CHEN Yu;TAN Weijun;CHENG Shujun(Guangzhou Chn？Alt Biotech.Co.,Ltd.,Guangzhou 510623,China;Guangdong Inspection and QuarantineTechnology Center,Guangzhou 510623;Guangdong Pharmaceutical University,Guangzhou 510006)
出处
《中国比较医学杂志》
CAS
北大核心
2018年第5期114-118,共5页
Chinese Journal of Comparative Medicine
基金
广州经济技术开发区国际科技合作项目(编号:2017GH11)
关键词
皮肤致敏
树突状细胞
替代方法
有害结局通路
skin sensitization
dendritic cells
DCs
alternative methods
adverse outcome pathway
AOP
