摘要
采用聚乙烯醇(PVA)为主要载体,添加少量壳聚糖(SA)、SiO_2和CaCO_3,利用聚乙烯醇-硼酸(PVA-H_3BO_3)包埋法,包埋苯酚降解菌TX1;并制备成胶珠,研究胶珠对含酚废水的降解。通过正交实验、单因素实验和传质性能分析,确定并优化包埋条件。结果表明PVA浓度8%、SA浓度0.2%、SiO_2浓度2.4%、CaCO_3浓度0.4%和饱和H_3BO_3液p H为6.7时,包埋胶珠机械强度好、结构稳定、无泄漏、固定化效果好;同时发现该条件下的固定化细胞培养12 h后对初始浓度为500 mg/L苯酚废水的降解率为78.1%。
Polyvinyl alcohol( PVA)-embedded beads were fabricated by Trichosporon sp. X1 immobilized with PVA-H3BO3 method using PVA as major carrier and additive with a little chitson,SiO2 and CaCO3. The degradation of phenol wastewater by PVA-embedded beads were investigated. With orthogonal test,single factor and masstransfer experiments,the best conditions for embedding strain TX1 were PVA 8%,SA 0. 2%,SiO2 2. 4% 、CaCO3 0. 3% and saturated H3BO3 which pH is 6. 7. PVA-embedded beads performed with high mechanical strength,structural stability,leak-tightness and good immobilization,which is well-suited for fluidized bed reactor. Finally,the degradation rate was 78. 1% when the concentration of phenol up to 500 mg/L after 12 h.
作者
吴芳芳
林皓
邱凌峰
胡家朋
徐婕
刘瑞来
WU Fang-fang;LIN Hao;QIU Ling-feng;HU Jia-peng;XU Jie;LIU Rui-lai(College of Ecological and Resource Engineering, Fujian Provincial Key laboratory of Eco-Industrial Green Technology, Wuyi University ,Wuyishan 354300, China;College of Environment and Resources, Fuzhou University , Fuzhou 350116, China;College of MaterialScience and Engineering, Fujian Normal University, Key Laboratory of Polymer Materials of Fujian Province , Fuzhou 350007, China)
出处
《科学技术与工程》
北大核心
2018年第14期209-214,共6页
Science Technology and Engineering
基金
国家自然科学基金(51406141)
福建省教育厅项目(JAT0595,JAT0596)
国家大学生创新性实验基金(201610397040,201710397016)
武夷学院基金(YJ201703,YJ201704)资助
关键词
包埋
丝孢酵母
降解
苯酚
embedded Trichosporon sp . degradation phenol