摘要
从罗汉果内生菌中筛选高产环糊精葡萄糖基转移酶(CGTase)的菌株进行菌种鉴定,并对其产酶条件进行优化。利用CGTase快速筛选法从罗汉果内生菌中筛选获得一株高产CGTase的菌株,编号为ND-6,经形态学和分子生物学鉴定其为一株解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。通过单因素和正交试验优化得到其产CGTase条件为1.0%环糊精作碳源,1.0%蛋白胨作氮源,初始p H值为8.0,装液量70 m L/250 m L,发酵温度40℃。在此优化条件下,菌株所产CGTase酶活达到9 753 U/m L,是优化前的1.43倍。
The high cyclodextrin glucosyltransferase （CGTase）-producing strains from endophytic bacteria in Siraitia grosvenorii was screened and identified, and its enzyme-producing conditions were optimized. Using CGTase rapid screening method, a strain with the high enzyme activity was obtained and named ND-6, and the strain was identified as Bacillus amyloliquefaciens by morphology and molecular biology. Through single factor and orthogonal experiments, the optimum CGTase-producing conditions were using cyclodextrin 1.0% as carbon source and peptone 1.0% as nitro-gen source, initial pH 8.0, loading volume 70 ml/250 ml, and fermentation temperature 40 ℃. Under the optimal conditions, the CGTase activity of the strain could be up to 9 753 U/ml, which was 1.43 times higher than that of before optimization.
作者
张昌志
范彩琴
龙楚媚
付强
赵丰丽
ZHANG Changzhi;FAN Caiqin;LONG Chumei;FU Qiang;ZHAO Fengli(l.College of Life Science,Guangxi Normal University,Guilin 541006, China;Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection,Ministry of Education,Guilin 541006, China)
出处
《中国酿造》
CAS
北大核心
2018年第5期97-102,共6页
China Brewing
基金
桂林市科学研究与技术开发计划项目(20171015-2)
关键词
罗汉果
内生菌
环糊精葡萄糖基转移酶
产酶条件
优化
Siraitia grosvenori
endophytic bacteria
cyclodextrin glucosyltransferase
enzyme-producing conditions
optimization
