摘要
目的研究上海市市售肉制品中沙门菌血清型、耐药谱和分子分型特征。方法 2016年1~8月在上海市所辖16个行政区,随机选择1个密集型社区,抽取1家超市或市场作为哨点,定期采集畜、禽类肉制品,按国家食品安全风险评估中心(CFSA)食源性疾病监测方案进行沙门菌分离、血清分型、抗生素定量检测与剂量评估、优势菌的分子分型。结果 606份样品包括猪、鸡、鸭、牛、羊、鹅肉制品,共分离沙门菌158株,总阳性率为26.1%,畜肉和禽肉来源分别占52.5%(83/158)和47.5%(75/158)。前5位沙门菌血清型依次为肠炎沙门菌、鼠伤寒沙门菌、德尔卑沙门菌、罗森沙门菌和印第安纳沙门菌,畜肉和禽肉来源优势菌型间差异有统计学意义(P<0.05);菌株对磺胺异噁唑耐药率最高(79.7%,126/158),对链霉素、萘啶酸、氨苄西林、四环素和氯霉素耐药率在38.0%~77.8%,多重耐药(MDR)菌株(≥3类)占77.8%(123/158),猪肉来源沙门菌对庆大霉素、链霉素、磺胺异噁唑、复方新诺明、氯霉素、四环素耐药率均高于鸡肉来源沙门菌,鸡肉来源沙门菌对头孢噻呋、头孢曲松、萘啶酸耐药率均高于猪肉来源沙门菌,差异均有统计学意义(P<0.05),32.9%(52/158)的菌株对至少6种抗生素超检测限值;肠炎沙门菌有15个分型,以1型和3型为优势型;鼠伤寒沙门菌有23个分型,具有遗传多样性特征。结论上海市市售肉制品沙门菌暴露以猪肉和鸡肉来源为主,肉制品中多重耐药沙门菌污染严重。猪肉来源的罗森沙门菌和鸡肉来源的吉韦沙门菌是新输入性的血清型。
Objective To find out the serotype distribution,molecular subtyping and drug resistance patterns of Salmonella isolates from marketed meat products in Shanghai. Methods A sentinel supermarket or market near an intensive community from each of 16 administrative regions in Shanghai were randomly selected,in which livestock and poultry meat products were collected regularly from January to August in 2016. Salmonella isolation and serotyping,antibiotic susceptibility testing, as well as molecular subtyping were conducted according to the foodborne disease surveillance monitoring program of the China National Center for Food Safety Risk Assessment. Results A total of 158 Salmonella strains were isolated from 606 commercially available meat products including pork,chicken,duck,beef,mutton and goose. Total positive rate was 26. 1%（ 158/606）,separation rates for livestock and poultry were 52. 5%（ 83/158） and 47. 5%（ 75/158） respectively. Salmonella enteritis, Salmonella typhimurium, Salmonella derby,Salmonella rissen and Salmonella indiana were the top 5 Salmonella serovars for meat products in Shanghai,but serovar type distributions in livestock and poultry were significantly different（ P 〈 0. 05）. Sulfisoxazole had the highest drug resistance rate for 158 Salmonella strains（ 79. 7%,126/158）,followed by streptomycin,nalidixic acid,ampicillin,tetracycline and chloramphenicol（ 38. 0% ~ 77. 8%）. Moreover,123 strains（ 77. 8%） were multi-drug resistant（ MDR）strains. Drug resistance rates of gentamicin, streptomycin, sulfonamides, trimethoprim/sulfamethoxazole,chloramphenicol,tetracycline and ceftiofur,ceftriaxone and nalidixic acid were significant different in Salmonella strainsfrom pigs and chicken（ P 〈 0. 05）. Six antibiotics exceeded the detection limits in almost half of the Salmonella strains.Salmonella enteritis strains had 15 PFGE patterns,in which type 1 and type 3 were predominant clones. Salmonella typhimurium strains had 23 significant different PFGE patterns,which showed genetic polymorphism features. Conclusion Salmonella contamination in retail meat and poultry is the main reason which leads to high Salmonella positive rate in Shanghai,the multidrug-resistant of Salmonella in meat products is serious and must be paid attention to. Salmonella rissen from pigs and Salmonella give from chicken are the newly imported animal originated Salmonella serovars.
作者
刘玥
顾其芳
刘诚
朱颖莹
刘弘
陈敏
许学斌
张红芝
LIU Yue, GU Qi-fang, LIU Cheng, ZHU Ying-ying, LIU Hong, CHEN Min, XU Xue-bin, ZHANG Hong-zhi(Municipal Center for Disease Control and Prevention, Shanghai 200336, Chin)
出处
《中国食品卫生杂志》
北大核心
2018年第2期132-138,共7页
Chinese Journal of Food Hygiene
基金
上海市卫生和计生委员会青年课题(20174Y0141)
健康危险因素监测与相关疾病预防控制(GWIV27.1)
关键词
沙门菌
血清型
多重耐药
分子分型
食源性致病菌
肉制品
上海
Salmonella
serovars
multi drug resistance
molecular subtyping
foodborne pathogenic bacteria
meat products
Shanghai
