摘要
目的探讨肿瘤坏死因子α-308（TNFα-308）位点基因多态性与慢性阻塞性肺疾病（COPD）的相关性。方法选取2014年2月至2017年3月在我院治疗的COPD患者143例，同时选取健康志愿者150例作为对照组，采用测序法检测2组TNFα-308位点基因多态性。结果TNF-α-308位点基因型共有3种：GG型、GA型和AA型；观察组基因型AA／GA和等位基因A频率分别为29．37％和17．13％，明显高于对照组的12．00％和6．67％；TNF-α-308位点GA／AA基因型频率与COPD患者性别、年龄、吸烟及严重程度无明显关系（χ2=0．001、0．009、0．035、0．001，P值均〉005）；GA／AA基因型患者支气管壁厚度、支气管壁厚度与肺动脉壁比值分别为（0．30±0．11）cm和0．40±0．12，明显高于GG型患者（t=11．231、6．667，P值均〈0．05），GA／AA型和GG型患者第1秒用力呼气容积占用力肺活量的百分比比较，差异无统计学意义（t=-0．525，P〉0．05）。结论TNF-α-308位点基因可能与COPD的易感性有关，其中A等位基因可能是易感基因，患者支气管壁厚度明显增加，但该位点多态性与COPD患者性别、年龄、严重程度等无关。
Objective To investigate correlation of tumor necrosis factor-α-308 （TNF-α 308） loci gene polymorphism and chronic obstructive pulmonary disease （COPD）. Methods One hundred and forty-three COPD patients treated in our hospital from February 2014 to March 2017 were selected, and 150 healthy volunteers were selected as control group. The two groups of TNFa-308 loci gene polymorphism were detected by sequencing. Results TNF-α-308 loci genotype were three： GG type, GA type and AA type. The frequencies of genotype AA/GA and allele A in the observation group were 29.37% and 17.13%,which were significantly higher than 12.00% and 6.67% in the control group. The genotype frequencies of TNF-α-308 loci gene GA/AA were not significantly related to the sex, age, smoking and severity of COPD patients （ χ2 = 0. 001,0. 009,0. 035,0.001, all P 〉0.05）. The bronchial wall thickness, ratio of bronchial wail thickness and pulmonary artery wall in GA/AA genotype patients were （0.30±0.11） cm and 0.40±0.12, significantly higher than those in type GG patients （ t = 11. 231, 6. 667, both P 〉0.05）. There was no significant difference in forced expiratory volume in the first second/ forced vital capacity in patients with type GA/AA and GG （ t = 0.525, P 〉0.05）.Conclusions TNF α- 308 loci gene may be associated with the susceptibility to COPD, the A allele may be a susceptible gene, the bronchial wall thickness is increased significantly, however, the polymorphism of this locus is not related to the sex, age and severity of COPD patients.
作者
余国辉
舒美玲
Yu Guohui, Shu Meiling(Department of Respiratory Medicine, Jingmen First People＇s Hospital, Jingmen 448000, Chin)
出处
《国际呼吸杂志》
2018年第9期648-651,共4页
International Journal of Respiration
