摘要
【目的】为了研究桃树花芽越冬过程中细胞内含物变化与花芽休眠的关系。【方法】以‘中华寿桃’(Prunus persica L.var.densa Makino cv.Zhonghuashoutao)为试材,分析比较越冬过程中8个时期花芽休眠状态和细胞内含物变化。【结果】‘中华寿桃’花芽在内休眠期间SOD活性呈现逐渐上升,随着内休眠解除,活性降低;POD活性在内休眠阶段出现先升高后降低的变化,内休眠解除过程中先降低后升高的变化;内休眠维持和解除过程中,CAT活性逐渐降低,而Vc出现几次较大的波动;内休眠阶段H_2O_2含量和产生速率出现先升高后降低的变化趋势,内休眠解除过程中维持相对平稳的状态;内休眠期间可溶性蛋白含量先降低后增加,而总氨基酸含量呈现先增加后降低的变化,内休眠解除过程中可溶性蛋白含量逐渐降低,总氨基酸含量呈逐渐上升的变化趋势;可溶性糖和脯氨酸含量越冬休眠期间总体呈现上升的趋势。【结论】‘中华寿桃’花芽可能在感受低温反应后,抗氧化代谢物质、可溶性糖和脯氨酸等细胞内含物发生变化,可能对其内休眠维持和解除产生一定作用。
[Purpose]Our aim was to study the relationship between the changes of cell inclusions and flower buds dormancy during the winter. [Method]Dormancy status and the changes of cell inclusions were analyzed and compared in flower buds of Prunus persica L. var. densa Makino cv.Zhonghuashoutao at eight different periods during the winter dormancy. [Results] The results indicated that SOD activity gradually increased during the period of endodormancy, and decreased with endodormancy release. POD activity increased at first and then decreased in the stage of endodormancy, and firstly descended and then increased in the progress of endodormancy release. CAT activity gradually decreased and Vc content appeared several large fluctuations in the process of endodormancy maintenance and release. The variation tendency of H2 O2 content and production rate were firstly up and then down in the stage of endodormancy, and kept at a relatively stable state during the endodormancy release. The content of soluble protein was firstly down and then up, and that of total amino acids was firstly up and then down during the period of endodormancy. However, soluble protein content gradually reduced and total amino acid content gradually rose in the process of endodormancy release. The contents of soluble sugar and proline showed an upward trend during the winter dormancy on the whole. [Conclusion]The results showed the contents of cell inclusions（antioxidant metabolism substances, soluble sugar, and proline, and other substances） in flower buds of ‘Zhonghuashoutao＇peach changed after low temperature reaction. It may have a certain effect on the maintenance and release of endodormancy.
作者
刘国琴
何嵩涛
刘进平
张睿
曾靖雯
LIU Guoqin;HE Songtao;LIU Jinping;ZHANG Rui;ZENG Jingwen(College of Tobacco Science of Guizhou University, Guiyang 550025, China;College of Forestry of Guizhou University, Guiyang 550025, China;College of Agriculture of Guizhou University, Guiyang 550025, China)
出处
《云南农业大学学报（自然科学版）》
CSCD
北大核心
2018年第1期90-98,共9页
Journal of Yunnan Agricultural University:Natural Science
基金
国家自然科学基金项目(31560537)
贵州省科学技术基金项目(黔科合J[2015]2040号)
关键词
中华寿桃
花芽
越冬休眠
细胞内含物
Prunus persica L. var. densa Makino cv. Zhonghuashoutao
flower bud
overwintering dormancy
cell inclusion
