摘要
研究鸢尾的内生真菌及其发酵物的抑菌活性和抗氧化活性,旨在深入开发新资源。以鸢尾为研究对象,分离纯化出内生真菌,采用DPPH自由基清除法和羟基自由基清除法对其发酵物进行抗氧化活性试验,采用牛津杯法和MTT法进行抑菌活性试验。结果表明:(1)采用组织分离法从鸢尾组织中分离出18株内生真菌;(2)菌株B-1-23、B-1-25、B-1-28和B-1-55具有较强的清除DPPH自由基的能力,菌株B-1-9、B-1-16和B-1-55具有较强的清除羟基自由基的能力;(3)菌株B-1-23、B-1-16、B-1-25、B-1-55和B-1-28的发酵物具有一定的抑菌作用,其中对枯草芽孢杆菌的抑制作用最强,1000μg/m L浓度下其抑菌率大于90%;对藤黄微球菌的抑制作用最弱,最高抑菌率为16.39%;(4)菌株B-1-55经分子生物学鉴定其为Fusarium镰刀属。试验结果说明鸢尾中蕴含着具有抑菌活性和抗氧化活性的内生真菌,可进一步开发利用。
To isolate the endophytic fungi of Iris tectorum and study the antibacterial and antioxidant activities of their fermentation products in order to find novel resources. The endophytic fungi were isolated and purified from Iris tectorum. The antioxidant activity test was carried out by DPPH radical scavenging method and hydroxyl radical scavenging method. Antibacterial activity test was carried out by Oxford cup method and MTT method. The results showed that：（1） 18 strains of endophytic fungi were isolated and purified.（2） Strains B-1-23, B-1-25, B-1-28 and B-1-55 had strong ability to scavenge DPPH free radicals. Strains B-1-9, B-1-16 And B-1-55 had the ability to scavenge hydroxyl radicals.（3） Strains B-1-23, B-1-16, B-1-25, B-1-55 and B-1-28 had some antibacterial activities. Among them, the inhibitory effect on Bacillus subtilis was the strongest, and the inhibitory rate was more than 90% at 1000 μg/m L. the inhibitory effect on Micrococcus lutea was the weakest and its highest antibacterial rate was 16.39%.（4） Strains B-1-55 was molecularly identified as Fusarium sp. The test results showed that the Iris contained endogenous fungi with antibacterial activity and antioxidant activity, which could be further exploited and utilized.
作者
毛涵
代飞
钱婷
汤新慧
薛菲
MAO Han1,2, DAI Fei1, QIAN Ting1, TANG Xin-hui1, XUE Fei1(1 .Jiangsu Provincial Key Laboratory of Saline Bioresources, Yancheng 224051; 2.College of Biotechnology and Pharmaceutical Engineering, Nanjing Technology University Nanjing 21181)
出处
《食品科技》
CAS
北大核心
2018年第5期19-25,共7页
Food Science and Technology
基金
国家自然科学基金项目(41101235)
江苏省盐土生物资源研究重点实验室开放课题(JKLBS2013010)
盐城师范学院自然科学基金项目(16YCKLQ003)
国家级大学生创新训练计划项目(201610324003)
江苏省"青蓝工程"科技创新团队项目
关键词
鸢尾
内生真菌
抗氧化
抑菌活性
Iris tectorum
endophytic fungi
antioxidant
antimicrobial activity
