摘要
目的初步探讨巨噬细胞极化在小鼠泡型包虫病疾病进展中的作用。方法建立小鼠泡型包虫病模型。于感染后90天处死模型小鼠收集血清标本和腹腔巨噬细胞(感染组),同时收集健康小鼠的血清和腹腔巨噬细胞(对照组)。分别应用流式细胞技术和ELISA法检测M1/M2巨噬细胞及相关因子的表达情况。结果 1.流式细胞技术结果示,与正常小鼠(0.25%±0.13%)相比,感染泡球蚴90天的小鼠CD16/32^+(24.18%±3.60%)的巨噬细胞(M1)百分比呈增加趋势,P<0.05;与正常小鼠(0.30%±0.14%)相比,感染泡球蚴90天的小鼠CD206^+(5.75%±0.45%)的巨噬细胞(M2)百分比呈增加趋势,P<0.05。2.ELISA结果显示,与正常对照组相比,感染组中IL-6、TNF-α、TGF-β1的含量均有不同程度增加,P<0.05;而感染组与对照组中IL-10差异不明显,P>0.05。结论极化后的M1/M2巨噬细胞参与了泡球蚴感染过程,二者的失衡可能导致了该疾病的进展。
Objective To study the preliminarily role of maerophage polarization in the development of alveolar hydatid disease in mice.Methods The mice model of alveolar hydatid disease was established by intraperitoneal in- jection of Echinococcus multiloeularis Protoscolex.The mice were sacrificed at 90 days post-infection to collect ser- um samples and peritoneal macrophages for the infection group, while healthy mice were collected serum and perito- neal macrophages specimens for the control group.Results 1.The results of flow eytometry showed an increase in the percentage of CD16/32＋ macrophages（ M1 ）in mice at 90 days post-infection（24.18%±3.60% ）compared to normal mice （0.25%±0.13% ）, P〈0.05. Moreover, The percentage of CD206＋ macrophages （M2）in mice at 90 days post-in- fection（5.75%＋0.45%） increased compared with the normal mice （ 0.30%±0.14%）, P〈0.05.2. The results of ELISA showed that the levels of IL-6, TNF-α and TGF-β1 in the infection group increased at different degrees re- spectively compared with those in the normal control group （P〈0.05） ;The difference of IL-10 was not obvious, P〉0.05.Conclusion M1/M2 macrophages are involved in the process of Echinococcus multilocularis infection,and the imbalance between them may lead to the progression of the disease.
作者
王东旭
王虎
樊海宁
王海久
王志鑫
汤锋
廖梦姣
王强
WANG Dong-Xu;WANG Hu;FAN Hai-ning;WANG Hai-jiu;WANG Zhi-Xin;TANG Feng;LIAO Meng-Jiao;WANG Qiang(Department of Hepatopancmatobiliary Surgery,Affihated Hospital of Qinghai University,Xining;Qinghai Province Key Laboratory of Hydatid Disease Researsh, Xining, Qinghai;Qinghai ,Qinghai Province Province, China Province Health ＆ Family Planning Commission, Xining, Qinghai Province, China;Research Center for High Altitude Medical Sciences, Qinghai Province, China)
出处
《中国高原医学与生物学杂志》
CAS
2018年第2期118-122,共5页
Journal of Chinese High Altitude Medicine & Biology
基金
青海省科技厅项目(2014-ZJ-719)
关键词
小鼠
泡型包虫病
巨噬细胞极化
免疫逃逸
Mice
Alveolar hydatid disease
Macrophage polarization
Immune escape
