摘要
高蛋白材料匮乏及选育方法单一是辽宁省高蛋白大豆育种中的两个突出问题。为探索高蛋白大豆种质资源创新有效途径,本研究以辽宁省农业科学院选育的辽04Q086、辽08020、辽05086和辽06Q003高蛋白品系为母本,与河北省沧州市农林科学院选育的高蛋白品种沧豆10号进行有性杂交,并对亲本及杂交F_2代进行不同剂量的辐射诱变处理,分析了不同剂量辐射诱变对不同受体的籽粒蛋白质和脂肪含量的改良效果。结果表明:4个大豆品系与沧豆10号杂交衍生的F_3代植株中,低蛋白高脂肪类型所占比例最高,达到了45.0%。4个大豆品系经辐射诱变(^(60)Coγ-14000和^(60)Coγ-16000)衍生的M_1代中,低蛋白高脂肪类型所占比例也分别达到42.5%和40.0%。然而,以杂交F_2代种子为受体进行不同剂量辐射诱变衍生的M_1代植株中,高蛋白低脂肪类型所占比例最高,分别为35.0%和27.5%,蛋白质含量最高达47.0%。本研究表明,以不同遗传背景的高蛋白大豆杂交,并结合辐射诱变选育高蛋白材料是可行的。
The scarcity of high-protein germplasm and singular breeding method are the two limiting factors for high-protein soybean breeding in Liaoning province. In order to explore an efficient innovation pathway of high-protein germplasm breed-ing, this study analyzed the change of protein and oil content in the generations of different receptors induced by different radi-ation doses. Four lines （LiaoO4Q086, Liao08020, Liao05086 and Liao06Q003) released in Liaoning province were used to cross with high-protein cuhivar Cangdou 10 released in Hebei province, and then the parents along with their crossing genera-tions were induced by 60Coγ ray. The results showed that the percentage of low-protein with high-oil types was 45.0%, which was larger than those of other types in the F3 generations. The similar results found in M1 generations from radiation treatment on four parents indicated that it was difficult to obtain high-protein types as lines being receptors. However, after radiation treatment on the F2 generations, the high-protein with low-oil types had a larger percentage （35.0% and 27.5%) than other types in their M1 generations, and the highest protein content reached 47. 0%. Results of this manuscript provided a feasible pathway to improve protein content of soybean through combining cross technique of high-protein parents with different background and radiation-induced mutation technique.
作者
董丽杰
李盛有
王雅珍
宋书宏
王文斌
曹永强
DONG Li-jie;LI Sheng-you;WANG Ya-zhen;SONG Shu-hong;WANG Wen-bin;CAO Yong-qiang(Institute of Crop Research, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China)
出处
《大豆科学》
CAS
CSCD
北大核心
2018年第3期337-341,共5页
Soybean Science
基金
国家大豆产业技术体系(CARS-004-CES11)
国家重点研发计划项目(2016YFD0100201-01
2017YFD0101304-2)
辽宁省中央引导地方科技发展专项(2017108006)
关键词
大豆
杂交
遗传背景
辐射诱变
品质
Soybean
Genetic background
Radiation-induced mutation
Quality
