摘要
目的了解手足口病密切接触者肠道病毒携带情况,探讨手足口病密切接触者在手足口病传播中的作用。方法 2016年3月~2016年10月采集手足口病密切接触人群咽拭子标本200份。咽拭子提取核酸后应用实时荧光RT-PCR技术检测人肠道病毒(HEV)、肠道病毒71型(EV71)和柯萨奇病毒A16型(CA16),按照医务人员与家属进行病毒检测情况分析。结果 200份标本中,HEV通用引物阳性104份(52.0%),其中EV71、CA16和其他肠道病毒阳性标本分别为43份(21.5%)、46份(23.0%)和15份(7.5%)。家属肠道病毒携带率高于医务人员,差异有统计学意义(P<0.01)。结论手足口病患儿密切接触者肠道病毒携毒率较高,其在手足口病的传播中意义更大。
Objective To investigate the intestinal virus carrying of the close contacts with hand-foot-and-mouth disease.To explore the role of close contacts with hand-foot-and-mouth disease in the transmission.Methods The throat swab samples from the close contacts with hand-foot-and-mouth disease were collected from March 2016 to October 2016. The HFMD pathogens which contain human enteroviruses（HEV）,enterovirus type 71（EV71） and coxsackievirus group A type 16（CA16） were detected by real-time fluorescent quantitative PCR.The carried rate was compared beteen the medical staff and family members.Results In 200 samples, HEV universal primer was detected52.0 %（104/200）.EV71 was detected in 21.5 %close contacts,while CA16 and other enteroviruses in 23.0 % and in 7.5% of the cases,respectively. The viruses carrier rate in family members was significantly higher than the carrier rate in medical staff（P〈0.01）. Conclusions The enteroviruses carrier rate of the close contacts with hand-foot-and-mouth disease is high.The close contacts who have been infected may play an important role in the spread of hand-foot-andmouth disease.
作者
屈丹丹
宋相安
刘霞
QU Dandan;SONG Xiangan;LIU Xia(Department of lnfectious Diseases of Puyang People＇s Hospital, Puyang, Henan457000,Chin)
出处
《河南预防医学杂志》
2018年第6期425-427,共3页
Henan Journal of Preventive Medicine
关键词
手足口病
密切接触者
携带率
肠道病毒
Hand-foot-and-mouth disease
Close contacts
Carrier rate
Enterovirus
