摘要
目的:探析不同煎煮方式对小柴胡汤中黄岑有效化学成分的影响。方法:选取高效液相色谱法测定小柴胡汤用去滓再煎法与不去滓煎法2种煎煮后的黄岑有效化学成分(黄岑苷、黄岑素、汉黄岑素)的含量。对照品溶液包括黄岑苷、黄岑素、汉黄岑素,检测开始前先进行对照品溶液的线性关系考察及精密度试验、稳定性试验、回收率试验。结果:黄岑苷、黄岑素、汉黄岑素对照品溶液的标准曲线方程分别为Y=4 345 730.98X-8 888.11(r=0.999 8)、Y=2 478 268.68X911.05(r=0.999 5)、Y=771 988 9.74X2 449.03(r=0.999 9)。黄岑苷、黄岑素、汉黄岑素对照品溶液的精密度试验、稳定性试验与回收率试验的RSD均低于2%,平均回收率均高于95%。供试品溶液A中所测得的黄岑苷、黄岑素、汉黄岑素含量分别为(0.364 2±0.027 1)g/L、(10.192 3±0.387 5)g/L、(18.082 2±0.746 5)μg/m L,高于供试品溶液B的(0.282 4±0.047 8)g/L、(8.215 0±0.354 1)g/L、(12.698 2±0.642 9)μg/m L(P<0.05)。结论:去滓再煎法较之传统不去滓煎法更利于小柴胡汤中黄岑有效化学成分黄岑苷、黄岑素、汉黄岑素的溶出。
Objective： To study the effects of different decoction methods on the effective chemical composition of Scutellaria baicalensis Georgi in Xiaochaihu Decoction. Methods： The contents of effective chemical composition（ Baicalin,Baicalein,Wogonin） in Xiaochaihu decoction boiled by deslagging and non-deslagging and then decocting were determined by high performance liquid chromatography. The comparison solution included the Baicalin,Baicalein and Wogonin. The linear relationship of the comparison solution and the precision test,the stability test and the recovery rate test were carried out before the start of the test. Results： The standard curve equation of Baicalin,Baicalein and Wogonin comparison solution were Y = 4 345 730. 98 X888 8. 11（ r =0. 999 8）,Y = 247 826 8. 68 X911. 05（ r = 0. 999 5） and Y = 771 988 9. 74 χ244 9. 03（ r = 0. 999 9）. The RSD of Baicalin,Baicalein,Wogonin reference solution in the precision test,stability test and the recovery test were all lower than 2% and the average recoveries were all higher than 95%. The contents of Baicalin,Baicalein and Wogonin in test solution A were（ 0. 364 2 ±0. 027 1） mg/m L,（ 10. 192 3 ± 0. 387 5） mg/m L and（ 18. 082 2 ± 0. 746 5） μg/m L,which were higher than（ 0. 282 4 ±0. 047 8） mg/m L,（ 8. 215 0 ± 0. 354 1） mg/m L and（ 12. 698 2 ± 0. 642 9） μg/m L in test solution B（ P 〈0. 05）. Conclusion：The deslagging and then decocting were more beneficial to the dissolution of Baicalin,Baicalein,Wogonin in Xiaochaihu decoction compared to the non-deslagging and then decocting.
作者
陈露露
林汝秀
李瑞明
Chen Lulu;Lin Ruxiu;Li Ruiming(Department of Pharmacy, Nanfang Hospital of Southern Medical University, Guangzhou 510515, China;Department of Pharmacy, Seventh Affiliated Hospital of Zhongshan University, Shenzhen 518107, China)
出处
《世界中医药》
CAS
2018年第3期743-745,750,共4页
World Chinese Medicine
基金
广东省重大科技专项项目(2011A080300004)
关键词
小柴胡汤
煎煮
方式
黄岑
有效
化学成分
Xiaochaihu decoction
Decoction method
Scutellaria baicalensis Georgi
Effective chemical composition
