摘要
目的探讨巨噬细胞和肺微血管内皮细胞对烟曲霉作用。方法构建小鼠巨噬细胞株J774细胞烟曲霉感染的体外模型和人肺微血管内皮细胞(HPMVECs)烟曲霉感染的体外模型,利用激光共聚焦扫描显微镜观察共培养体系。结果 J774细胞对烟曲霉具吞噬作用,但在共培养24 h的动态观察中,并未观察到HPMVECs内化烟曲霉,仅观察到烟曲霉的感染导致HPMVECs形态的变化和细胞完整性的破坏。结论 J774细胞对烟曲霉具吞噬作用,HPMVECs无内化烟曲霉的作用,烟曲霉感染可导致HPMVECs形态的变化和细胞完整性的破坏。
Objective To investigate effect of macrophages and pulmonary microvascular endothelial cells（HPMVECs）on Aspergillus fumigatus. Methods In vitro models of Aspergillus fumigatus-treated HPMVECs and Aspergillus fumigatus-treated J774 cells were established, confocal laser scanning microscope was used for detection of co-cultures. Results Aspergillus fumigatus were phagocyted by J774 cells. However, Aspergillus fumigatus were not internalized by HPMVECs, onlu morphology change and integrity compromise were observed on Aspergillus fumigatus-treated HPMVECs. Conclusion J774 cells phagocytes Aspergillus fumigatus. HPMVECs can＇t internalize Aspergillus fumigatus.Aspergillus fumigatus can cause morphology change and integrity compromise in HPMVECs.
作者
宋珺
韩菁
孙越
施伟民
SONG Jun;HAN Jing;SUN Yue;SHI Weimin(Department of Dermatology, Shanghai First People＇s Hospital, Shanghai Jiaotong University, Shanghai 200080, Chin)
出处
《中国医药导报》
CAS
2018年第13期18-20,F0003,F0004,共5页
China Medical Herald
基金
国家重点基础研究发展计划(973计划)(2013CB531604)
关键词
巨噬细胞
肺微血管内皮细胞
烟曲霉
Macrophages
Pulmonary microvascular endothelial cells
Aspergillus fumigatus
