摘要
目的:探讨温肾养血方提高子宫内膜容受性的作用机制。方法:建立肾虚血瘀型胚胎着床障碍大鼠模型,运用SP免疫组化法、蛋白质印迹法和实时荧光定量PCR检测正常组,模型组,高、中、低剂量组血管内皮生长因子(VEGF)、血管内皮生长因子受体2(KDR)的表达情况。结果:与模型组比较,温肾养血方高剂量组的VEGF、KDR蛋白表达均显著升高(P<0.05)。结论:温肾养血方能促进子宫内膜VEGF和KDR的表达,并且其效果与剂量成正相关,提示温肾养血方提高妊娠率的作用机制可能与通过调节子宫内膜血流改善子宫内膜容受性有关。
Objective： To explore the mechanism of Wenshen Yangxue Decoction（WSYXD） to improve endometrial receptivity. Methods： The dysfunctional implantation rat model with kidney deficiency and blood stasis was made. Comparing the vascular endothelial growth factor（VEGF）, KDR expressions among normal group, model group and high, middle, and low dose WSYXD group by SP immunohistochemistry, Western Blot and q-PCR. Results： Compared with the model group, the protein expressions of VEGF and KDR in the high dose WSYXD group increased, and the difference was statistically significant（P〈0.05）. Conclusion： WSYXD can promote the expression of VEGF and KDR in endometrium, and the effect is positively related to the dose. WSYXD may improve pregnancy rate by regulating the endometrial blood flow.
作者
辛明蔚
何军琴
武颖
梁欣韫
张莹
杨维
尹晓丹
XIN Ming-wei;HE Jun-qin;WU Ying;LIANG Xin-yun;ZHANG Ying;YANG Wei;YIN Xiao-dan(Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2018年第6期2386-2389,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金青年科学基金项目(No.81503607)
北京市中医药科技发展资金青年研究项目(No.QN2014-02)
北京市优秀人才培养资助项目(青年骨干个人)(No.2014000021469G247)
北京市医院管理局重点医学专业发展计划(No.ZYLX201510)~~
