摘要
目的探讨has-miR-155(Homo sapiens miR-155,miRNA-155)在食管癌组织中的表达,分析其表达与患者临床病理特征的关系。方法收集食管癌标本80例,另取患者癌旁组织作为对照组。采用RT-PCR检测食管癌组织及癌旁组织中miR-155表达水平,分析miR-155表达水平与患者临床病理特征以及临床分期的关系。结果 RT-PCR结果显示,食管癌组织中miR-155的表达水平为0.437±0.091,与癌旁组织相比差异具有统计学意义(t=25.184,P=0.007)。食管癌组织中miR-155的表达与患者性别和年龄无关(P>0.05);与食管癌组织学分级,肿瘤浸润深度,淋巴结转移和TNM分期均有一定的关系(P<0.05)。结论 miR-155在食管癌的发生,发展中起着重要作用,对食管癌潜在的治疗靶点及预后判断具有重要意义。
Objective To investigate the expression of has-miR-155（ Homo sapiens miR-155,miRNA-155） in esophageal and its relationship with clinicopathological features. Methods 80 cases of esophageal cancer specimens were treated with thoracic surgery,and the adjacent tissues were taken as the control group. The expression of miR-155 in esophageal carcinoma tissues and adjacent tissues was detected by RT-PCR. The relationship between the expression of miR-155 and clinicopathological features and clinical stage was analyzed. Results The expression of miR-155 in esophageal cancer tissues was 0. 437 ± 0. 091,which was statistically significant（ t = 25. 184,P = 0. 007）. The expression of miR-155 in esophageal carcinoma was analyzed by immunohistochemistry. The expression of miR-155 in esophageal carcinoma was not correlated with sex and age of patients（ P 〉0. 05）. The histological grade,depth of tumor invasion,lymph node metastasis and TNM staging were correlated（ P 〈 0. 05）.Conclusion miR-155 plays an important role in the occurrence and development of esophageal carcinoma,which is of great significance to the potential therapeutic target and prognosis of esophageal cancer.
作者
聂龙
周方正
童琳
潘东风
张东升
王小聪
谢辉
郭思言
NIE Long;ZHOU Fangzheng;TONG Lin(Suizhou Hospital,Hubei University of Medicine,Suizhou,441300)
出处
《实用癌症杂志》
2018年第6期878-880,共3页
The Practical Journal of Cancer
