摘要
从草甘膦污染土壤中分离获得一株耐受400 mmol/L草甘膦的菌株S1536,该菌株在草甘膦浓度为100~400 mmol/L之间生长迅速,最适pH为6.0,最适生长温度为30℃,具有氨苄青霉素抗性。以S1536基因组DNA为模板,通过通用引物进行扩增、测序获得其16S rDNA序列,在Gen Bank登录号为MG519831,在NCBI中经BLAST比对与泛菌属(Pantoea sp.)同源性达到99%。用ClustalW对S1536与泛菌属各个种模式菌的16S rDNA进行多重序列对比,该菌株与Pantoea rodasii同源性最为接近,达到99.2%,故将菌株S1536命名为Pantoea rodasii S1536。该菌株具有较高草甘膦抗性,对温度和pH适应性强,是优良的耐草甘膦菌株,通过全基因组测序及基因注释,获得抗除草剂基因aroA序列,为下一步明确基因功能,挖掘Pantoea rodasii S1536高抗草甘膦的分子机制奠定基础。
A 400 mmol/L glyphosate-resistant strain S1536 was isolated from the glyphosate polluted soil. The strain grew well in the medium with glyphosate concentration between 100 - 400 mmol/L. The optimal pH was 6.0,optimal growth temperature was 30℃,and had resistance to ampicillin. Taking S1536 genomic DNA as a template,this experiment carried out amplification and sequencing and gained 16 S rDNA sequence,which registration number was MG519831 in Gen Bank. Compared with the published nucleotide sequence of 16 S rDNA in NCBI（ National Center for Biotechnology Information）,S1536 showed 99% homology with Pantoea sp. Multiple sequence comparison between S1536 and the model bacteria of Pantoeasp of 16 S rDNA was conducted by ClustalW. This strain showed high identity with Pantoea rodasii,reaching 99. 2%,so it was named as Pantoea rodasii S1536. The strain possessed higher glyphosate resistance and good adaptability to temperature and pH. It was a superior glyphosate tolerant strain. aroA sequence resistant to herbicide was obtained through whole genome sequencing and gene annotation,which provided a solid foundation for further identification of gene function and exploitation of the molecular mechanism of high glyphosate resistance of Pantoea rodasii S1536.
作者
杨小艳
刘亚娟
吴红
王忠伟
雷开荣
谢树章
YANG Xiaoyan;LIU Yajuan;WU Hong;WANG Zhongwei;LEI Kairong;XIE Shuzhang(Biotechnology Research Center, Chongqing Academy of Agricultural Sciences, Chongqing 401329;Chongqing Energy College, Chongqing 402260, China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2018年第6期47-54,共8页
Journal of Agricultural Science and Technology
基金
重庆市农业科学院良种创新暨重大科技推广项目(NKY-2016AA003-2)
重庆市基础科学与前沿技术研究一般项目(cstc2017jcyjAX0395)
重庆市科研院所绩效激励引导专项(cstc2017jxjl80001)资助
关键词
草甘膦抗性
鉴定
生长特性
泛菌属
glyphosate resistance
identification
growth characteristics
Pantoea sp.
