摘要
目的:探讨四逆软肝方对高浓度乙醛培养的人肝星状细胞肝纤四项(CG、HA、LN、PCIII)及基质金属蛋白酶1(MMP-1)、组织金属蛋白酶抑制剂1(TIMP-1)的影响。方法:采用高浓度乙醛对人肝星状细胞LX-2株造成损伤后,随机分为四逆软肝方、清热解毒化浊片、扶正化瘀片和脱离乙醛4组;经给药24h后搜集各组细胞培养液上清,采用ELISA试剂盒检测肝纤四项及MMP-1、TIMP-1含量。结果:250mmol/L乙醛培养72h,细胞存活率最高;与正常组比,乙醛组CG、HA、LN、MMP-1、TIMP-1差异无统计学意义(P>0.05),PCIII升高(P<0.01)。与正常组比较,脱离乙醛组CG下降(P<0.01)、PCIII升高(P<0.01);与脱离乙醛组比较,四逆软肝方组HA、PCIII、TIMP-1均降低(P<0.01),CG、LN、MMP-1差异无统计学意义(P>0.05)。四逆软肝方组、清热解毒化浊片组、扶正化瘀片组组间比较,差异无统计学意义(P>0.05)。结论:四逆软肝方可降低受损的LX-2细胞HA、PCIII、TIMP-1指标的表达,可能对抗肝纤维化有一定的作用。
Objective： To investigate the effect of Sini Ruangan prescription on four liver fibrosis markers[cholyglycine（ CG）,hyaluronic acid（ HA）,laminin（ LN）,and procollagen III（ PCIII） ],metal matrix proteinase-1（ MMP-1）,and tissue inhibitor of metalloproteinase-1（ TIMP-1） in human hepatic stellate cells cultured in high-concentration acetaldehyde. Methods： After LX-2 human hepatic stellate cells were injured by a high concentration of acetaldehyde,these cells were randomly divided into Sini Ruangan prescription group,Qingre Jiedu Huazhuo tablets group,Fuzheng Huayu tablets group,and acetaldehyde-removed group. At 24 hours after administration,cell culture supernatants were collected from all groups,and ELISA was used to measure the levels of four liver fibrosis markers,MMP-1,and TIMP-1. Results： The cells cultured in 250 mmol/L acetaldehyde for 72 hours had the highest survival rate. There were no significant differences in the expression of CG,HA,LN,MMP-1,and TIMP-1 between the normal group and the acetaldehyde group（ P〉0. 05）,and compared with the normal group,the acetaldehyde group had a significant increase in PCIII（ P〈0. 01）. Compared with the normal group,the acetaldehyde-removed group had a significant reduction in CG and a significant increase in PCIII（ both P〈0. 01）. Compared with the acetaldehyde-removed group,the Sini Ruangan prescription group had significant reductions in HA,PCIII,and TIMP-1（ P〈0. 01）,while there were no significant differences in CG,LN,and MMP-1 between the two groups（ P〉0. 05）. There were no significant differences between the Sini Ruangan prescription group,the Qingre Jiedu Huazhuo tablets group,and the Fuzheng Huayu tablets group（ P〉0. 05）. Conclusion： Sini Ruangan prescription can reduce the expression of HA,PCIII,and TIMP-1 in injured LX-2 cells and thus exerts an anti-liver fibrosis effect.
作者
赵国荣
唐菲
梅明
邹俊驹
刘近明
李凌云
肖碧跃
Jiri Horak
ZHAO Guo - tong;TANG Fei;MEI Ming;ZOU Jun - ju;LIU Jin -ming;LI Ling - yun;XIAO Bi - yue;Jiri Horak(Hunan University of Chinese Medicine, Changsha 410208, Hunan, China;Third Faculay by of medicine charls University prague Czech)
出处
《湖南中医杂志》
2018年第5期177-180,共4页
Hunan Journal of Traditional Chinese Medicine
基金
湖南省财政厅科学技术厅国际合作科技计划项目(编号:2015WK3019)
湖南中医药大学中医临床基础重点学科资助项目
关键词
四逆软肝方
高浓度乙醛
人肝星状细胞
肝纤四项
MMP-1
TIMP-1
实验研究
Sini Ruangan prescription
high - concentration acetaldehyde
human hepatic stellate cell
four liver fibrosis markers
metal matrix proteinase - 1
tissue inhibitor of metalloproteinase - 1
experimental study
