摘要
目的探讨孕期小鼠双酚A暴露对子代雌鼠青春期发育提前及对下丘脑-垂体-性腺轴(HPG轴)激素水平的影响。方法 105只8周龄清洁级CD-1小鼠分为对照组和双酚A低、中、高剂量组,从受孕0~18天分别给予双酚A 0、8、40和200mg/kg。子代出生后进行性别判断和称重。出生后21~34天,观察雌性子代阴道开口情况,并通过阴道涂片判定动情周期。仔鼠于出生后第34天处死并取血,检测血清促性腺激素释放激素(GnRH)、卵泡刺激素(FSH)、促黄体生成素(LH)、雌二醇(E2)水平。结果孕期BPA低、中、高剂量组的子代雌鼠阴道开口时间分别为(28.0±0.36)、(28.0±0.33)和(28.0±0.61)d,早于对照组的(30.0±0.27)d,差异均有统计学意义(P<0.05);阴道开口提前率均高于对照组,第一次动情时间均早于对照组,差异均有统计学意义(P<0.05);GnRH水平分别为(9.78±1.07)、(11.55±1.38)和(10.09±1.51)pg/mL,均高于对照组[(5.18±4.63)pg/mL],而低剂量组LH水平为(1.86±0.79)pg/mL,低于对照组[(2.37±1.56)pg/mL],差异均有统计学意义(P<0.05)。结论孕期母鼠双酚A暴露其子代雌鼠青春期发育明显提前,同时增加子代雌鼠GnRH激素水平,影响HPG轴功能。
Objective To investigate the effect of maternal exposure to bisphenol A on puberty in advance and hypothalamus-pituitary-gonad axis（ HPG axis） hormones level in female offspring. Methods A total of 105 pregnant CD-1 mice aged 8 weeks were daily administered with BPA at four different doses（ 0,8,40 and 200 mg/kg） by gavage from gestational day（ GD） 0 to 18. Offspring were weighed and determined sex by anal reproductive distance after birth. From postnatal day（ PND） 21-34,the vaginal opening of female offspring and distinguished the estrous stage through vaginal smears wereobserved. The level of GnRH,FSH,LH and E2 hormone in serum were detected.Results The mean initial time of vaginal opening in 8 mg/kg group [（ 28. 0 ± 0. 36）days],40 mg/kg group [（ 28. 0 ± 0. 33） days] and 200 mg/kg group [（ 28. 0 ± 0. 61）days]was earlier than that in the control group [（ 30. 0 ± 0. 27） days]. The vaginal opening in advance rate of BPA exposure group was higher than that of the control group and the first estrus occurrence was earlier than controls（ P〈0. 05）. The levels of GnRH[8 mg/kg：（ 9. 78 ± 1. 07） pg/mL,40 mg/kg：（ 11. 55 ± 1. 38） pg/mL,200 mg/kg：（ 10. 09 ± 1. 51） pg/mL]with increased expressions compared with the controls [（ 5. 18± 4. 63） pg/mL]. LH level in low dose group [（ 1. 86 ± 0. 79） pg/mL] was significantly lower than that in control group [（ 2. 37 ± 1. 56） pg/mL]. The above differences were statistically significant（ P〈0. 05）. Conclusion These findings suggested that maternal exposure to BPA result in advancing puberty and increase the GnRH hormone level to affect the function of HPG axis in female offspring.
作者
徐耿
韩阿珠
许诺
苏普玉
Xu Geng;Han Azhu;Xu Nuo;Su Puyu(Department of Maternal, Child and Adolescent Health, School of Public Health, Anhui Medical University, Hefei 230032, Chin)
出处
《卫生研究》
CAS
CSCD
北大核心
2018年第3期425-431,共7页
Journal of Hygiene Research
基金
国家自然科学基金(No.81573163
8110214)
安徽医科大学第三批中青年学术骨干资助基金(2012)
关键词
双酚A
下丘脑-垂体-性腺轴
青春期发育提前
动物实验
环境毒理
bisphenol A
hypothalamo-pituitary gonadal axis
puberty in advance
animal experiment
environmental toxicology
