摘要
【目的】蜡蚧菌Lecanicillium是自然界一类重要的昆虫病原真菌,为了获取更多的蜡蚧菌作为控虫真菌资源,对从森林中的根际土壤分离得到的一株蜡蚧菌进行鉴定以及与其他几种病原真菌进行烟草粉斑螟Ephestia elutella的致病性对比。【方法】采用形态特征比较和转录间隔区(ITS)序列构建分子系统树对从贵州贵定松林下土壤中分离的菌株进行综合鉴定,采用浸虫法对烟草粉斑螟3龄幼虫进行致病性筛选。【结果】根据形态特征比较和系统发育分析,该真菌为丝枝蜡蚧菌Lecanicillium aphanocladii。在测试菌株中,环链棒束孢Isaria cateinannulata、球孢白僵菌Beauveria bassiana和丝枝蜡蚧菌对烟草粉斑螟3龄幼虫7 d的校正死亡率分别为79.22%,64.92%和77.92%,表现出较好的致死效果。【结论】丝枝蜡蚧菌对烟草粉斑螟幼虫的致死效果较好,可用作仓储内烟草粉斑螟的生物防治资源。
【Aim 】 Lecanicillium is one group of important fungal pathogens. In this study,a strain separated from the rhizosphere soil in the forest was identified and its pathogenicity to Ephestia elutella was compared with those of some other fungal pathogens so as to acquire more Lecanicillium strains as pest control fungal resources. 【Methods 】 The fungal colonies isolated from the soil under pine forests in Guiding,Guizhou were identified by comparison of morphological characteristics and internal transcribed spacer（ ITS） sequence analysis. The pathogenecity of the test strains to the 3 rd instar larvae of E.elutella was determined by the method of immersing insects into the spore suspension. 【Results 】According to the morphological characterization and phylogenetic tree, the fungi was identified as Lecanicillium aphanocladii. Among the test strains,Isaria cateinannulata,Beauveria bassiana and L.aphanocladii were lethal to E. elutella,with the corrected mortality rates of 79. 22%,64. 92% and77. 92%,respectively, against the 3 rd instar larvae at 7 d after treatment. 【Conclusion 】 L.aphanocladii,a known species in China,shows high lethal effect to E. elutella larvae,and can be usedfor biological control of E. elutella larvae in the warehouse.
作者
周叶鸣
郅军锐
张鑫
邹晓
杨凯
ZHOU Ye-Ming;ZHI Jun-Rui;ZHANG Xin;ZOU Xiao;YANG Kai(Institute of Entomology, Guizhou University, Guiyang 550025, China;Institute of Fungal Resource, Guizhou University, Guiyang 550025, China;Shanghai Tobacco Group Limited Liability Company, Shanghai 200082, China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2018年第4期505-510,共6页
Acta Entomologica Sinica
基金
上海烟草集团有限责任公司科技项目(0721-1564485HB409)
贵州省农业科技攻关项目(黔科合NY[2013]3034)
黔科合重大专项字[2016]3022-07号
关键词
蜡蚧菌
丝枝蜡蚧菌
烟草粉斑螟
致病性
生物防治
虫生真菌
Lecanicillium
Lecanicillium aphanocladii
Ephestia elutella
pathogenicity
biologicalcontrol
entomogenous fungi
