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Box-Benhnken设计-响应面法优化四氧化三铁固定化酶催化葡萄糖酸锌合成工艺研究 被引量:3

Box-Behnken Design for Optimization of the Synthesis Process for Zinc Gluconate by Enzyme Immobilized on Fe3O4
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摘要 以D-葡萄糖和氧化锌为原料,在四氧化三铁固定化葡萄糖氧化酶催化下,用简便方法合成葡萄糖酸锌,并通过单因素试验确定各因素的合理范围。以反应温度、缓冲溶液pH、D-葡萄糖体积量为考察因素,以葡萄糖酸锌的产量为因变量,通过Box-Benhnken设计-响应面法优化葡萄糖酸锌制备工艺条件。根据建立的各因素与响应值之间的数学模型,确定最佳制备工艺条件为反应温度38℃、缓冲溶液pH 7.3、D-葡萄糖体积量3 mL,获得葡萄糖酸锌量的最高值为406.16 mg,与模型预测值偏差0.58%。结果表明,固定化酶法制备葡萄糖酸锌工艺简单、成本较低,具有较高的工业应用价值。 Using D-glucose and zinc oxide as raw materials,zinc gluconate was synthesized by a simple method catalyzed by immobilized glucose oxidase of Fe_3O_4.The reasonable range of each factor was determined by a single factor test. Taking the reaction temperature,buffer solution pH,and D-glucose volume as the investigated elements,the yield of zinc gluconate was optimized by the Box-Behnken design-response surface method,using the yield of zinc gluconate as a dependent variable.The Box-Behnken design utilizing three factors and three levels of response surface experiment,a mathematical model of each factor,and a response value determined the best synthesis process.The results show that various factors have an influence on the synthesis of zinc gluconate.The best synthesis conditions are 38 ℃,pH 7. 3,and 3 mL Glc.The results show that the preparation of zinc gluconate by an immobilized enzyme method is simple,low cost,and has high industrial application value.
作者 杜会茹 黄文杰 杨雷 于文国 DU Hui-ru;HUANG Wen-fie;YANG Lei;YU Wen-guo(Department of Pharmarceutical Engineering, Hebei Chemical and Pharmarceutical College, Shijiazhuang 050018, China)
出处 《化学试剂》 CAS 北大核心 2018年第6期537-541,共5页 Chemical Reagents
基金 河北省科技厅重点基础研究项目(15962801D) 河北省教育厅青年基金项目(QN2017053)
关键词 葡萄糖酸锌 四氧化三铁 固定化酶 响应面法 zinc gluconate Fe3O4 enzyme immobilization response surface method
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