摘要
选用Chlorella pyrenoidosa FACHB-5和Chlorella vulgaris FACHB-8为试验藻种,利用人工气候培养箱,在培养温度为(26±1)℃、光照强度为4 000 lx、通入空气流量为1.5 L/min、24 h连续光照、沼液添加量为20%的条件下,采用中温35℃和高温55℃猪粪厌氧发酵后沼液为原料,研究两种沼液灭菌和不灭菌处理后与BG11培养基混合作为微藻培养液对后续微藻培养过程的影响。研究结果表明,高温55℃厌氧发酵后的沼液用于微藻培养的整体表现优于中温35℃厌氧发酵后沼液,微藻的生长速率更高。在本研究的条件下,沼液灭菌组与未灭菌组相比,在前期的适应期方面未表现出明显的优势,但在中后期的微藻生长速率方面略优于未灭菌组。本研究所选定的两种藻种均能较好地适应添加沼液的培养环境,并且Chlorella pyrenoidosa FACHB-5的整体表现优于Chlorella vulgaris FACHB-8。
Nutrient-rich biogas slurry containing N and P is potential culture media for microalgae. Some researchers also conducted a series of relative studies. However, the effect of different biogas slurry on the subsequent microalgae culture is significantly different because the miscellaneous germs in biogas slurry can inhibit the microalgae growth. Furthermore, the variety and quantity of bacteria in biogas slurry digested at medium temperature (35℃) and high temperature (55℃) are also significantly different. However, fewer systematic comparison of biogas slurry digested at medium temperature (35℃) and high temperature (55℃) is found based on microalgae culture. Therefore, the Chlorella pyrenoidosa (FACHB -5) and the Chlorella vulgaris ( FACHB - 8) were employed as algae species in this experiment. The effect of broth being the culture medium ( BG11 ) mixed with the autoclaved and non-autoclaved biogas slurry of pig manure after anaerobic digestion at 35℃ and 55℃ on following microalgae growth was studied by using artificial climate incubator. The specific culture temperature, light intensity, continuous light period, air flow and additive ratio of biogas slurry were (26 ± 1 ) ℃ , 4 000 lx, 24 h, 1.5 L/min and 20% , respectively. The results indicated that the effect of hiogas slurry digested at 55℃ was better than that of biogas slurry digested at 35℃ , and the growth rate of microalgae obvious cultured in the biogas slurry of anaerobic fermentation at 55℃ was more superior. Moreover, there was no difference in early adaptation period of microalgae growth between autoclaved biogas slurry and non-autoclaved biogas slurry. However, the growth rate of microalgae cultured in autoclaved biogas slurry was slightly optimal in middle and late period. What' s more, the algae species used in this experiment could adapt to the culture environment with biogas slurry addition well. The superior to that of Chlorella vulgaris (FACHB - growth of Chlorella pyrenoidosa ( FACHB - 5 ) was 8). The microalgae also decreased the ammonium nitrogen, total nitrogen, total phosphorus and chemical oxygen demand (COD) in biogas slurry significantly, which was favorable for the purification and comprehensive utilization of biogas slurry. The research provided a theoretical basis for the subsequent research and application of microalgae culture with biogas slurry.
作者
王丽丽
司爱龙
李泽
WANG Zhiwu
隋超
王忠江
WANG Lili;SI Ailong;LI Ze;WANG Zhiwu;SUI Chao;WANG Zhongjiang(College of Engineering, Northeast Agricultural University, Harbin 150030, China;Department of Civil and Environmental Engineering, Virginia Polytechnic Institute and State University, Blacksburg VA 24061, USA)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2018年第6期325-331,共7页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家高技术研究发展计划(863计划)项目(2014AA022001)
国家自然科学基金项目(51406032)
教育部留学回国人员科研启动基金项目(20131792)
中央引导地方科技发展专项项目(ZY17C05)
关键词
猪粪沼液
中温
高温
灭菌
微藻
厌氧发酵
pig manure biogas slurry
medium temperature
high temperature
autoclaved
microalgae
anaerobic digestion