摘要
以封丘金银花为材料,利用高效液相色谱法建立同时测定金银花不同器官(花、叶、茎)中绿原酸、咖啡酸、木犀草苷3种活性成分含量的检测方法。并以清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基和ABTS^+·自由基的能力为评价指标,对金银花不同器官的抗氧化活性进行比较。结果表明,建立的高效液相色谱方法实现了对3种活性成分的分离,且线性关系良好(R^2≥0.9997),3个器官中3种活性成分的平均回收率在89.45%~102.35%之间。该方法简便、准确,可靠性强。含量测定结果为:绿原酸在花中含量最高,为27.22 mg·g^(-1),其次是茎(19.68 mg·g^(-1))和叶(12.80 mg·g^(-1));木犀草苷在茎中含量最高,为2.31 mg·g^(-1),其次是叶(1.66 mg·g^(-1))和花(1.07 mg·g^(-1));咖啡酸在花、叶和茎的含量分别是1.18、1.05、0.93 mg·g^(-1)。3个器官中,以花的提取物抗氧化能力相对较强,清除DPPH和ABTS^+·自由基的IC_(50)分别为0.07 mg·m L^(-1)和0.05 mg·m L^(-1),茎和叶具有一定的抗氧化活性,两种评价方法均表明,抗氧化能力大小依次是花>茎>叶。
Lonicerae japonicae Thunb.was used as material to establish the high performance liquid chromatography （HPLC） method for simuhaneously determining the content of chlorogenic acid, caffeic acid and galuteolin in different organs （ flowers, leaves and stems）of Lonicerae japonicae Thunb..The antioxidant activities of extracts from different organs of Lonicerae japonica Thunb.were evaluated through DPPH· and ABTS＋· free radical scavenging ability. Results showed that, three active components were separated by the HPLC method with a good linear relationship （R2 ≥ 0.9997 ） and average recovery rates ranged from 89.45% to 102.35% .Therefore, the method was simple, accurate and highly reliable. The content of chlorogenic acid in the flowers was 27.22 mg·g-1 ,followed by the stems（ 19.68 mg·g-1 ）and the leaves（ 12.80 mg·g-1 ）.The content of luteolin in the stems was the highest,which was 2.31 mg·g-1 ,followed by leaves（ 1.66 mg·g-1） and flowers（ 1.07 mg·g-1 ）.The contents of caffeic acid in the flowers,leaves and stems were 1.18,1.05,0.93 mg·g-1, respectively.The extracts from flowers showed the highest antioxidant activity to DPPH· and ABTS ＋ · among three organs with IC50 0.07 mg·mL-1 and 0.05 mg·mL-1 , and the extracts of stems and leaves also showed antioxidant capacity to some extent.Antioxidant capacity order was flowers 〉 stems 〉 leaves.
作者
徐小博
徐萍
毛雪飞
李俊峰
任敏
XU Xiao-bo;XU Ping;MAO Xue-fei;LI Jun-feng;REN Min(College of Life Science and Technology, Xinxiang University, Xinxiang 453003, Chin)
出处
《食品工业科技》
CAS
CSCD
北大核心
2018年第13期41-45,共5页
Science and Technology of Food Industry
基金
河南省科技攻关项目(152102310018)
新乡学院博士科研启动项目(1366020055)
关键词
金银花
不同器官
绿原酸
木犀草苷
咖啡酸
高效液相色谱法
抗氧化活性
Lonicerae japonicae Thunb.
different organs
chlorogenic acid
galuteolin
caffeic acid
HPLC
antioxidant activity
