摘要
目的了解南通市活禽养殖及销售环境中禽流感的携带情况及流行规律,为监控和防治人感染禽流感疫情提供基础资料。方法采集活禽养殖场及交易市场外环境标本,运用RT-PCR技术检测甲型流感病毒核酸并分型。结果 602份外环境样本中,检出甲型流感阳性样本89份,阳性率为14.8%;其中33份样本可同时检出2种型别,合并感染的样本中以H7N9和H9N2合并感染居多,共20份,占合并感染总数的60.6%。65份样品中检出H7N9,40份样品中检出H9N2,13份样品中检出H9N9,4份样品中检出H5N1。多种家禽混合样本中禽流感检出率较高,宰杀或摆放禽肉案板表面的擦拭样本以及清洗禽类的污水中检出率较高。结论 H7N9和H9N2为当前本地区禽流感主要型别,流行季节以冬春季为主,对禽类市场、家庭养殖户和养殖场的管控仍需加强。
[Objective]To explore the carrying status and epidemic law of avian influenza（AI） virus in live poultry breeding and sales environment in Nantong City,provide basic information for monitoring and preventing human infection with avian influenza.[Methods] The external environment samples were collected from live poultry farm and trading market,and RT-PCR technology was used to detect influenza A nucleic acid followed by subtyping. [Results] There were 89 positive samples for influenza A among 602 external environment samples with a positive rate of 14.8%. 33 samples were positive for two or more subtypes,and most of co-infections（20 cases） were H7 N9 and H9 N2,accounting for 60.6% of total co-infection cases. H7 N9 was detected in65 samples,H9 N2 in 40,H9 N9 in 13,and H5 N1 in 4. The detection rate was high in mixed samples from different poultry.Surface wipe samples from slaughtering meat boards and sewage samples from cleaning poultry also made the detection rates much higher. [Conclusion] Influenza A H7 N9 and H9 N2 are the principal epidemic subtypes of avian influenza in Nantong City.The epidemic seasons are winter and spring. Surveillance and control on living poultry markets,breeding farmers and poultry farms should be strengthened.
作者
苏婧
季霄雷
郭陈
熊海平
SU Jing;Jl Xiao-lei;GUO Chen;XIONG Hai-ping(Department of Microbial Testing, Nantong Center for Disease Control and Prevention, Nantong Jiangsu, 226007, Chin)
出处
《职业与健康》
CAS
2018年第11期1471-1474,共4页
Occupation and Health
基金
2017年度江苏省人兽共患病学重点实验室开放课题(R1703)
