摘要
目的研究黄芪甲苷(AS-IV)对秀丽隐杆线虫寿命的影响,并探索其在干预秀丽隐杆线虫衰老过程中代谢物的变化规律。方法分别收集对照组、衰老模型组和AS-IV组秀丽隐杆线虫样本,采用~1H-NMR代谢组学技术及多元统计分析方法研究秀丽隐杆线虫组织内源性代谢产物的变化。结果 5μmol/L的AS-IV可以显著升高衰老线虫组织中8种氨基酸的含量,包括异亮氨酸、亮氨酸、缬氨酸、谷氨酸、谷氨酰胺、琥珀酸、牛磺酸和苏氨酸,同时可降低葡萄糖的含量。结论 5μmol/L的AS-IV能够通过干预机体氨基酸代谢、能量代谢等发挥延缓衰老的作用。代谢组学方法为进一步研究AS-IV延缓衰老的作用机制提供了科学依据。
Objective To investigate the anti-aging effects and the changes of endogenous metabolites in Caenorhabditis elegans by using astragaloside IV（AS-IV）. Methods Nematodes tissue were collected from control group, age group and AS-IV group to analyze the metabolic profile changes among different groups by nuclear magnetic resonance（NMR）. Results Using 1 H-NMR metabonomics and multivariate statistical analysis methods, the changes in the levels of endogenous metabolites in the model group C. elegans were obtained, including eight increasing amino acids isoleucine, leucine, valine, glutamate, glutamine, succinate, taurine and threonine and decreasing compound glucose, compared with the control group. AS-IV（5 μmol/L） can significantly regulate all of them. Conclusion AS-IV can regulate amino acid metabolism and energy metabolism to anti-aging. The metabonomic approach is an effective tool for the investigation of the pharmacologic mechanism of AS-IV and it is helpful for further research.
作者
薛晓利
张建琴
马雯
李震宇
秦雪梅
XUE Xiao-li;ZHANG Jian-qin;MA Wen;LI Zhen-yu;QIN Xue-mei(Modem Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, Chin)
出处
《中草药》
CAS
CSCD
北大核心
2018年第10期2274-2281,共8页
Chinese Traditional and Herbal Drugs
基金
山西省高等学校科技创新项目(2015119)
山西省科技创新重点团队(201605D131045-18)
山西省应用基础研究项目(201601D202058)
关键词
黄芪甲苷
秀丽隐杆线虫
寿命
衰老
代谢组学
astragaloside Ⅳ
Caenorhabditis elegans
lifespan
aging
metabonomics
