摘要
目的建立一种同时定性、定量测定胃复春片中柚皮苷、人参皂苷Re,Rg_1,Rb_1,Rf,Rb_2,Rg_2,Rd,20(R)-Rg_3、冬凌草甲素和表诺多星醇11种成分含量的高效液相-质谱联用法(HPLC-MS)。方法用电喷雾离子化源,正负离子多反应监测模式建立含量测定方法。色谱柱:Eclipse XDB C_(18)柱(150 mm×4.6 mm,5μm)流动相:乙腈-0.5‰甲酸,梯度洗脱,流速:0.7 m L·min^(-1)。考察该方法的专属性、标准曲线和定量下限、精密度与回收率、稳定性和重现性。结果胃复春片中待测11个化学成分在15 min内完全分离,方法的线性关系良好,11种成分的平均相关系数r=0.999 0。线性范围、精密度及稳定性较好。平均回收率在94.24%~99.93%,日内和日间精密度均不超过7.0%,相对标准偏差不超过4.10%。结论该方法简便、准确、重现性好、专属性高,能同时测定胃复春片中11种成分的含量,可用于胃复春片的质量控制。
Objective To develop a simultaneous analysis method for 11constituents including naringin,ginsenoside Re,Rg1,Rb1,Rf,Rb2,Rg2,Rd,20（R）-Rg3,oridonin and epinodosinol in Wei-Fu-Chun tablets by HPLC-MS.Methods The compounds were detected using the positive ion multiple-reaction monitoring mode by MS with electrospray ionization.Chromatographic separation was performed on a C18HPLC column,with gradient elution of 0.5‰formic acid aqueous solution and acetonitrile as mobile phase at a flow rate of 0.7 m L·min^-1.Results The 11 components in Wei-Fu-Chun tablets could be well separated in 15 min.There was significant correlation（r=0.999 0）between the ratio of peak area and the concentration of each compound within the test ranges.The average recoveries of the 11 compounds were in the range of 94.24%to 99.93%with the relative standard deviations less than 4.10%.Conclusion The method is simple,rapid,accurate and with good reproducibility and can be used for the determination of 11components in Wei-Fu-Chun tablets.
作者
张红霞
张丽芳
张莉
冯梅梅
刘丽华
ZHANG Hong - xia;ZHANG Li - fang;ZHANG Li;FENG Mei -mei;LIU Li - hua(Department of Pharmacy, Fourth Hospital of Shijiazhuang, Shijiazhuang 050000, China;Z The Biomedical Engineering Center, Hebei Medical University, Shijiazhuang 050000, Chin)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2018年第12期1470-1474,共5页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81402894)
河北传统中药材管理基金资助项目(C2011206158
08B031)
